中国药理学通报2011，Vol.27Issue(10)：1458-1462,5.DOI:10.3969/j.issn.1001-1978.2011.10.029
姜黄素在大鼠体内药代动力学和生物利用度研究
Pharmacokinetics and absolute bioavailability of curcumin in rats
张立康 1汪小珍 2李婉姝 1邱相君 3孙未 1胡国新1
作者信息
- 1. 温州医学院药学院,浙江,温州,325035
- 2. 温州医学院附属第二医院,浙江,温州,325027
- 3. 河南科技大学医学院,河南,洛阳,471003
- 折叠
摘要
Abstract
Aim To study the pharmacokinetics and absolute bioavailability of curcumin in rats with different administration.Methods A HPLC method was developed for the determination of curcumin in rat plasma.Oral, intraperitoneal and ranine vein doses of 200,20 mg · kg-1 an 10 mg · kg-1 were respectively administered, and the concentrations of curcumin were determined with HPLC.The pharmacokinetic parameters were calculated with the program DAS 2.0.The absolute bioavailability of curcumin was calculated according to AUC(0-∞ ) and the doses of curcumin following oral, intravenous and intraperitoneal administration.Results Excellent linear relationship was obtained in the range of 0.05 ～ 6.00 mg · L-1( r =0.9998 ).The lower limit determination of curcumin was 0.05 mg · L-1.The relative recoveries were ( 99.29 ±5.40 )％,( 104.21 ±4.72 )％ and( 99.83 ±1.97 )％ respectively at three concentrations ( 0.10 mg · L-1, 1.00 mg ·L-1, 4.00 mg · L-1 ).The intraday RSD were 4.49％ , 3.90％ and 1.72％.The interday RSD were 4.61％ , 4.27％ and 2.00％ respectively.The metabolic processes of curcumin in rats all fit in with the two-compartment model following oral,intravenous and intraperitoneal administration.Elimination half life were ( 159.28 ± 18.12 ), ( 90.79 ±11.55 ), ( 11.96 ± 2.64 ) min respectively and AUC(0-∞) were ( 86.36 ± 12.90 ) mg · min · L-1,( 73.39 ±8.72 ) mg · min · L-1, ( 104.62 ± 11.89 )mg · min · L-1 respectively.The absolute bioavailability of intraperitoneal administration was 35.07％and the absolute bioavailability of intragastric administration was 4.13％.Conclusion With different administration, the pharmacokinetics process of curcumin is similar in rats.The absolute bioavailability of intraperitoneal administration is relatively high, while the absolute bioavailability of intragastric administration is low.关键词
姜黄素/腹腔给药/灌胃给药/消除半衰期/绝对生物利用度/高效液相色谱法/药代动力学Key words
curcumin / intraperitoneal administration / intragastric administration/ elimination half life/ absolute bioavailability/ HPLC/ pharmacokinetics分类
医药卫生引用本文复制引用
张立康,汪小珍,李婉姝,邱相君,孙未,胡国新..姜黄素在大鼠体内药代动力学和生物利用度研究[J].中国药理学通报,2011,27(10):1458-1462,5.
