军事医学2011，Vol.35Issue(8)：611-615,5.
pp32在前列腺癌细胞中的作用研究
Effects of pp32 on prostate cancer cells
程倩 1张达矜 1刘冬梅 1吴祖泽 1崔春萍1
作者信息
- 1. 军事医学科学院放射与辐射医学研究所,北京,100850
- 折叠
摘要
Abstract
Objective To explore the effect of pp32 on prostate cancer cells. Methods The pcDNA3.1 and pcDNA3. 1-pp32 plasmids previously stored in our laboratory were transferred into Pc3M cells in order to construct stable Pc3M-pp32 cell lines. Morphological changes of Pc3M cells were observed by Giemsa staining experiments, and the cells ability of migration was measured through Transwell's chamber assays. The cells ability of adhesion was assessed by adhesion experiments, and MTT assays were used to determine the cell growth curve. Finally, the expression of N-cadherin was investigated by Western blotting. Results We successfully obtained Pc3M cell lines with pp32 over-expression ( Pc3Mpp32 ), and pp32 significantly induced morphological changes of Pc3M cells. Pp32 could also enhance the migration and adhesion of human prostate cancer cells Pc3M. Pp32 could ohviously increase Pc3M cell growth and effectively inhibit the expression of N-cadherin in Pc3M cells. Conclusion Over-expression of pp32 leads to morphological changes of Pc3M cells, and promotes the migration, adhesion, and growth of these cells.关键词
前列腺肿瘤/磷蛋白类/pp32/质粒/转染/Pc3M细胞/细胞运动/细胞黏附Key words
prostatic neoplasms / phosphoproteins/ pp32/ plasmids / transfection/ Pc3M cells / cell movement/ cell adhesion分类
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程倩,张达矜,刘冬梅,吴祖泽,崔春萍..pp32在前列腺癌细胞中的作用研究[J].军事医学,2011,35(8):611-615,5.
