渔业科学进展2011，Vol.32Issue(3)：44-50,7.
达氟沙星在健康大菱鲆体内的药代动力学研究
Pharmacokinetics of danofloxacin in turbot Scophthalmus maximus following intravenous and oral administration
摘要
Abstract
Danofloxacin, a third-generation member of the quinolone family, it has been also used widely to prevent or treat bacterial diseases in fish farming in many countries. It has been studied in Paralichthys olivaceus , Acipenser schrenckii , and goldfish Carassius auratus Linnaeus, etc. However, to our knowledge, there are no studies concerning the pharmacokinetics of danofloxacin in turbot Scophthalmus maximus. In this study, the pharmacokinetics behavior of danofloxacin in turbot after intravenous and oral administration was investigated.Six fish were randomly selected from the tank (water temperature 16 ±0.6℃) and sampled at 15, 30min, and 1, 2, 4, 6, 8, 12, 16, 24, 36, 48, 72 and 96h after intravenous and oral administration, respectively. The concentrations of danofloxacin were determined by HPLC. The data were analyzed with the pharmacokinetic program DAS 2.0. The results showed that the relationship of plasma concentration versus time for danofloxacin were best described as a twocompartment open model, and the pharmacokinetic equations were: Civ = 45. 741e-l.927t +7. 332e-0.019t, Cpo =6. 796e-0.05t+3. 135e-0.005t-9. 931e-0.11t. After the intravenous and oral administration, the main pharmacokinetic parameters were as follows: t1/2β 36. 336h and 129.228h, AUC0-96h 431. 981 and 284. 915 h · μg/ml, respectively. After oral administration, the concentration of danofloxacin in liver and kidney was higher than in muscle, but was similar with the concentration in plasma. According to the danofloxacin pharmacokinetics and the minimal inhibitory concentration of most fish bacterial pathogens, the dosage regime of danofloxacin for treatment of fish bacterial diseases was calculated as a daily dosage of 12.17mg/kg by oral administration.关键词
达氟沙星/大菱鲆/药代动力学Key words
Danofloxacin/ Scophthalmus maximus/ Pharmacokinetics分类
农业科技引用本文复制引用
梁俊平,李健,常志强,韩现芹,赵法箴..达氟沙星在健康大菱鲆体内的药代动力学研究[J].渔业科学进展,2011,32(3):44-50,7.基金项目
国家自然科学基金(30700617)和公益性农业行业专项(nyhyzx07-046)共同资助 （30700617）
