实用肝脏病杂志2011，Vol.14Issue(5)：321-322,326,3.DOI:10.3969/j.issn.1672-5069.2011.05.001
TAT-N24穿膜融合多肽对HepG2细胞增殖的影响
Inhibition of cell-permeable TAT-N24 fusion peptide on proliferation of HepG2 cells in vitro
摘要
Abstract
Objective To investigate the anti-proliferation effects of cell-permeable TAT-N24 fusion peptide in hepatoma cell line. Methods HepG2 cells were treated with cell-permeable TAT-N24 fusion peptide.The cell cycle was analyzed by flow cytometry,the proliferation of the cells was assayed by BrdU incorporation and the expression of phosphorylated AKT was detected by Western blot. Results The incorporation rates of BrdU in blank, control and TAT-N24 group were 42.7±3.6％,38.2±2.8％ and 25.3±2.7％ (P＜0.05) , respectively;the percentages of G0/G1 phase cells were 52.6±4.7％,52.0±4.6％ and 68.6±4.7％ (P＜0.05) , respectively; the phosphorylation of AKT in the three groups was not significantly different. Conclusion Cell-permeable TAT-N24 fusion peptide effectively inhibites the DNA synthesis and induces cell cycle blocked at G0/G1 phase of HepG2 cells.关键词
HepG2细胞/TAT-N24穿膜融合多肽/分子靶向治疗Key words
HepG2 cells/ Cell-permeahle TAT-N24 fusion peptide/ Molecular targeting therapy引用本文复制引用
邓豫,王桂华,金源,李小兰,陶德定,李维娜,龚建平,胡俊波..TAT-N24穿膜融合多肽对HepG2细胞增殖的影响[J].实用肝脏病杂志,2011,14(5):321-322,326,3.基金项目
国家973计划项目 (No.2009CB521802) （No.2009CB521802）
国家自然科学基金资助项目(No.30872472,30973496,30800569) （No.30872472,30973496,30800569）
湖北省自然科学基金资助项目(No.2008CDB174,2009CDB239) （No.2008CDB174,2009CDB239）
