中国组织工程研究与临床康复2011，Vol.15Issue(37)：6937-6941,5.DOI:10.3969/j.issn.1673-8225.2011.37.023
人颈椎间盘退变与细胞凋亡及基质金属蛋白酶11的表达
Matrix metalloproteinase 11 expression and cell apoptosis in human cervical disc degeneration
摘要
Abstract
BACKGROUND: Previous studies have shown that matrix metalloproteinase 11 gene is obviously upregulated in humandegenerated cervical and lumbar disc tissues.OBJECTIVE: To investigate the relationship between matrix metalloproteinase 11 expression in human cervical intervertebraldiscs and cell apoptosis.METHODS: Thirty discs from patients with cervical spondylosis diagnosed by MRI, and 20 discs of individuals with cervical spinaltrauma but without anamnesis evidence for disc degeneration or previous spine-ralated operations were included.RESULTS AND CONCLUSION: Hematoxylin-eosin staining showed that nucleus pulposus cells were signifcantly reduced(P ＜ 0.01), but apoptotic cells were significantly increased (P ＜ 0.01) in the degenerated nucleus pulposus tissue of cervicalintervertebral discs than in the normal nucleus pulposis tissue. Immunohistochemical staining showed that matrixmetalloproteinase 11 expression was significantly greater (P ＜ 0.01) in the degenerated nucleus pulposus tissue of cervicalintervertebral discs than in the normal nucleus pulposis tissue, and matrix metalloproteinase 11 expression was positively related to cell apoptosis detected by TUNEL(r=0.44, P ＜ 0.05). Results showed that high expression of matrix metalloproteinase 11 can directly damage extracellular matrix, induce nucleus pulposis cell apoptosis, and therefore play an important role in the process of disc degeneration.关键词
颈椎间盘/髓核/退变/细胞凋亡/基质金属蛋白酶11分类
基础医学引用本文复制引用
郭团茂,刘淼,张银刚,张小卫,武世勋..人颈椎间盘退变与细胞凋亡及基质金属蛋白酶11的表达[J].中国组织工程研究与临床康复,2011,15(37):6937-6941,5.基金项目
国家自然科学基金(30400163),课题名称:骨髓间质干细胞椎间盘内异位分化及差异基因的评价 （30400163）
陕西省科技计划项目(2008K14-01),课题名称:人退变颈椎间盘组织基因表达谱的分析. （2008K14-01）
