中国组织工程研究与临床康复2011，Vol.15Issue(37)：6972-6976,5.DOI:10.3969/j.issn.1673-8225.2011.37.031
递增负荷运动大鼠脑皮质神经元和脑脊液中去甲肾上腺素、5-羟色胺的动态变化
Dynamic changes of norepinephrine and 5-hydroxytryptamine in cerebral cortex neuron and cerebrospinal fluid of rats after movement with increasing loads
摘要
Abstract
BACKGROUND: Exercise-induced fatigue is a hot topic in the field of sports medicine, and whether there is some associationbetween exercise-induced fatigue occurrence and recovery and cerebrospinal fluid circulation remains poorly understood.OBJECTIVE: To study the dynamic changes and correlation of norepinephrine (NE) and 5-hydroxytryptamine (5-HT) in cerebralcortex and cerebrospinal fluid of rats after movement with increasing loads.METHODS: 30 male SD rats were evenly divided into control group (C), immediately after incremental exercise group (E1), 24hours after incremental exercise (E2). The control group rats were conventionally fed, and the E1 and E2 group rats received 6weeks of incremental exercise and then sacrificed immediately and 24 hours after exercise. Cerebrospinal fluid was extracted andcerebral cortex was taken. NE and 5-HT levels in cerebral cortex and cerebrospinal fluid were detected by high-performance liquidchromatography-fluorescence detection.RESULTS AND CONCLUSION: The NE and 5-HT levels in the cerebral cortex and cerebrospinal fluid in the E1 group weresignificantly higher compared with the C group (P ＜ 0.01). The NE and 5-HT levels in the cerebral cortex were significantly higherin the E2 group compared with the C group (P ＜ 0.01), but there were no significant differences in NE and 5-HT levels incerebrospinal fluid between the E2 group and the C group. In the E1 group, NE and 5-HT levels in the cerebral cortex weresignificantly positively correlated (P ＜ 0.05) and NE and 5-HT levels in the cerebral cortex and cerebrospinal fluid were highlycorrelated (P ＜ 0.01). Results showed that immediately and 24 hours after incremental exercise, the excited effect of NE and theinhibitory effect of 5-HT were in a dynamic balance and that immediately after incre mental exercise, the dynamic changes of NEand 5-HT in the cerebrospinal fluid can reflect the dynamic changes of NE and 5-HT in the cerebral cortex.关键词
运动性中枢疲劳/去甲肾上腺素/5-羟色胺/皮质/脑脊液分类
基础医学引用本文复制引用
杨澎湃..递增负荷运动大鼠脑皮质神经元和脑脊液中去甲肾上腺素、5-羟色胺的动态变化[J].中国组织工程研究与临床康复,2011,15(37):6972-6976,5.基金项目
成都体育学院院管课题(YJ0701). （YJ0701）
