中国组织工程研究与临床康复2011，Vol.15Issue(38)：7053-7056,4.DOI:10.3969/j.issn.1673-8225.2011.38.006
纤维蛋白胶外支架转染PCNA基因反义寡核苷酸预防移植静脉再狭窄
Effect of fibrin glue associated with antisense to PCNA on preventing restenosis of vein grafts
摘要
Abstract
BACKGROUND: Preliminary findings show that fibrin glue is not only a good non-restrictive, extravascular biodegradable stent,but also can prevent intimal and medial hyperplasia of vein grafts. It is also a good drug delivery system that can improve theextravascular membrane gene transfection efficiency.OBJECTIVE: To verify the effect of fibrin glue associated with antisense to PCNA on preventing restenosis of vein grafts.METHODS: Rabbit models of external jugular vein carotid artery bypass grafting were prepared and then randomized into modelgroup, fibrin glue group and fibrin glue+antisense group. Commercially available fibrin glue and fibrin glue mixed with adenovirusexpressing the antisense oligocleotides to PCNA were applied separately around vein grafts in the latter two groups, respectively.RESULTS AND CONCLUSION: Twenty-eight days after operation, the intimal and medial thickness and area was increasedobviously in the model group and decreased significantly in the fibrin glue group (P ＜ 0.01). A significant difference in the intimaland medial thickness and area was found between the fibrin glue group and fibrin glue+antisense group (P ＜ 0.05). The mRNAand protein expressions of PCNA in the fibrin glue+antisense group was lower than those in the fibrin glue group (P ＜ 0.05). Theexpression of PCNA in vein grafts can be inhibited by adventitial delivery of antisense to PCNA. The fibrin glue mixed withantisense has a synergistic effect on reducing the intimal and medial thickness and area of vein grafts.关键词
纤维蛋白胶/反义寡核苷酸/增殖细胞核抗原/移植静脉/再狭窄/外支架分类
基础医学引用本文复制引用
万力,王文俊,曹园平,王群,刘季春..纤维蛋白胶外支架转染PCNA基因反义寡核苷酸预防移植静脉再狭窄[J].中国组织工程研究与临床康复,2011,15(38):7053-7056,4.基金项目
国家自然科学基金资助项目(30760247 （）
30960380). （）
