中国组织工程研究与临床康复2011，Vol.15Issue(38)：7118-7122,5.DOI:10.3969/j.issn.1673-8225.2011.38.020
血管内皮生长因子与纳米晶胶原基骨支架复合修复大鼠股骨缺损
Vascular endothelial growth factor and nano-hydroxyapatite/collagen composite in the repair of femoral defect in rats
摘要
Abstract
BACKGROUND: Previous studies have confirmed that nano-hydroxyapatite/collagen (nHAC) and mesenchymal stem cells for repair of bone defect have the ability of bone formation in vivo. OBJECTIVE: To observe the effects of vascular endothelial growth factor (VEGF) and bone marrow mesenchymal stem cells (BMSCs), nHAC composite in the repair of femoral defect in rats. METHODS: Sprague-Dawley rat models of middle part of the femur defect were established and randomly assigned to two groups. Control group was implanted with BMSCs/nHAC composite. Experimental group was implanted with VEGF/BMSCs/nHAC composite. At 2, 4 and 8 weeks postoperation, imaging and histology observation of femoral samples were performed. At 8 weeks postoperation, scanning electron microscopy was performed in new bony callus environment. RESULTS AND CONCLUSION: nHAC composite implantation in the rats did not show rejection or inflammatory reaction. Moreover, bone formed rapidly using VEGF and BMSCs, nHAC composite, which exhibited better bone regeneration capacity compared with BMSCs/nHAC composite. The way of ossification mainly was endochondral ossification. It is presumed that VEGF promoted the formation of local microvessels, differentiation and proliferation of osteoblasts, speeded up the speed of endochondral ossification, shortened bone repair time, and elevated the quality and velocity of osteanagenesis.关键词
纳米晶胶原基骨/血管内皮生长因子/骨髓间充质干细胞/骨缺损/环境扫描电镜分类
医药卫生引用本文复制引用
徐成振,杨文贵,何晓峰,周立涛,韩雪昆,徐晓峰..血管内皮生长因子与纳米晶胶原基骨支架复合修复大鼠股骨缺损[J].中国组织工程研究与临床康复,2011,15(38):7118-7122,5.基金项目
the Social Development Foundation Program of Zhenjiang City,No.SH2002019 镇江市社会发展基金资助项目(SH2002019). （SH2002019）
