世界中医药2011，Vol.6Issue(5)：444-447,4.
平喘方及其拆方对支气管哮喘小鼠肺组织转录因子T-bet/GATA-3表达的影响
Effects of Ping Chuan Formula and its Disassembled Prescriptions on T bet/GATA 3 in Bronchial Asthmatic Model Mice
摘要
Abstract
Objective :To discuss effects and mechanism of Ping Chuan formula and its different disassembled formulae on preventing and curing asthma.Methods :To establish the model of asthmatic mice, the BALB/c mice were sensitized by intra-peritioneal injection of mixed ovalbumin and then activated by inhaling ovalbumin.120 BALB/c mice were randomly divided into 6 groups: the normal group,the asthma model group, the Ping Chuan formula group, CF 1 ( Ping Chuan formula disassembled prescription 1 group ), CF2, CF3.After the 7 days and 21 days of activated asthma, the lung cells were treated with Trizol for extraction of total RNA, the gene expression of T -bet, GATA -3 were analyzed by RT -PCR; the general histological changes of lung tissue were observed by hematoxylin and eosin stain.Results:At the 7th day, the expression of T - bet in CF1、 CF2 and CF3 were obviously increased( P ＜ 0.05 );the expression of GATA -3 in Ping Chuan formula、 CF2 and CF3 were obviously decreasedP ＜0.01 ).The ratio of T -bet/GATA -3 in CF3 were higher than the other goupe ( P ＜0.05 ).At the 21th day,the expression of T - bet were increased in every groupe( P ＜0.01 ),the expression of GATA - 3 significantly decreased( P ＜ 0.01 ) ,The ratio of T - bet/GATA - 3 were increased ( P ＜ 0.01 ).CF1 and CF2' s T - bet expression level were lower than Ping Chuan formula and CF3( P ＜ 0.05 ), GATA - 3 were higher ( P ＜ 0.01 ), and the ratio of T - bet/GATA - 3 were lower than Ping Chuan formula and CF3 ( P ＜0.05 orP ＜0.01 ).Conclusion:Ping Chuan formula and its different disassembled prescription can regulate the expression of T- bet/GATA -3 mRNA which are the upstream factors of Th1/Th2, then regulate the balance of Th1/Th2.CF3' s curative effect is nearly equal to the effect of Ping Chuan formula.关键词
支气管哮喘/中医药疗法/平喘方/T-bet/GATA-3/Th1/Th2Key words
Bronchial asthma/ Chinese medical therapy/ Ping Chuan formula/ T -bet/ GATA -3 /Th1/Th2引用本文复制引用
白莉,张新光,吴杰,朱慧华,赵毅涛,张晓峰,虞坚尔..平喘方及其拆方对支气管哮喘小鼠肺组织转录因子T-bet/GATA-3表达的影响[J].世界中医药,2011,6(5):444-447,4.基金项目
国家自然科学基金资助项目(30873286) （30873286）
上海市科委资助项目(074119625) （074119625）
