中国组织工程研究与临床康复2011，Vol.15Issue(38)：7066-7070,5.DOI:10.3969/j.issn.1673-8225.2011.38.009
3-羟基丁酸与3-羟基己酸共聚酯的血管内生物相容性
Intravascular biocompatibility of poly (3-hydroxybutyrate- co-3-hydroxyhexanoate)
吴松 1刘迎龙 2唐跃 2王强 2万峰 1曲向华 3陈国强3
作者信息
- 1. 北京大学第三医院心脏外科,北京市,100191
- 2. 中国医学科学院心血管病研究所,北京协和医学院阜外心血管病医院心血管外科,北京市,100037
- 3. 清华大学生物系微生物研究室,北京市,100084
- 折叠
摘要
Abstract
BACKGROUND: The degradable poly (3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) has superior mechanical property and biocompatibility.OBJECTIVE: To elucidate the intravascular biocompatibility of PHBHHx in vivo.METHODS: We developed hybrid materials based on decellularized xenogenic vascular scaffolds that were coated with PHBHHx and implanted it into the abdominal aorta of New Zealand rabbits. The decellularized xenogenic pulmonary artery patch without PHBHHx coating served as the control. The implanted patches were determined for the histology, immunofluorescence staining, scanning electron microscopy and calcium contents at 1, 4 and 12 weeks after the surgery.RESULTS AND CONCLUSION: Hybrid patches exhibited smooth lumen surface without thrombus, the intimal hyperplasia was mild and recellularization was complete; immunofluorescence staining showed that the endothelial cells in the neointima were positive for CD31, with continuous single-layer arrangement, interstitial cells were positive for smooth muscle actin; the calcium content in hybrid patches was obviously lower than that in uncoated patches. PHBHHx shows a remarkable intravascular biocompatibility in vivo and is believed as an ideal candidate for lumen coating of cardiovascular tissue engineering.关键词
脱细胞异种血管/3-羟基丁酸与3-羟基己酸共聚酯/血管内生物相容性/心血管组织工程分类
基础医学引用本文复制引用
吴松,刘迎龙,唐跃,王强,万峰,曲向华,陈国强..3-羟基丁酸与3-羟基己酸共聚酯的血管内生物相容性[J].中国组织工程研究与临床康复,2011,15(38):7066-7070,5.
