医药导报2011，Vol.30Issue(10)：1340-1343,4.DOI:10.3870/yydb.2011.10.029
中空纤维分离-高效液相色谱法测定复方痤疮凝胶中主药的含量
Separation and Determination of Clindamycin Phosphate and Tretinoin in Acne Gel by Hollow Fiber-High Performance Liquid Chromatography
马亚中 1吴燕 1刘丽平 1林向阳2
作者信息
- 1. 空军总医院药学部,北京,100142
- 2. 解放军空军后勤部卫生部,北京,100720
- 折叠
摘要
Abstract
Objective A simple sample pretreatment method was developed for the determination of clindanycin phosphate and tretinoin in Acne Gel by hollow fiber-HPLC. Methods The gel sample pretreatment was carried out by hollow fiber. The chromatographic analysis was carried out on a Venusil XBP-C18 column( 150 mm×4.6 mm,5 μm )with acetonitrile and potassium dihydrogen phosphate buffer solution( the pH value was adjusted to 3.0 by phosphoric acid )as the mobile phase bygradient elution at a flow rate of 1.0 mL · min-1. The column temperature was 30 ℃ and sample size was 20 μL. The detection wavelength of clindanycin phosphate and tretinoin in Acne Gel were set at 210 nm and 350 nm, respectively. Results The good resolution between peaks of main drugs and of the adjacent purity was obtained. Good linear relations in determination of clindamycin phosphate and tretinoin were obtained in the range of 48.00- 480. 00 μg · mL1( r = 0. 999 8 ) and 1.00-20.00 μg · mL-1 ( r=0.999 7 ),respectively. Meanwhile,the average recovery were 99.96％( RSD=0.69％ ,n=6 )and 99.99％ ( RSD = 1.24％ ,n = 6 ). Conclusion This method is simple and accurate, and it's suitable for the sample pretreatment and content determination of mian drugs in acne gel.关键词
痤疮凝胶/中空纤维/含量测定/色谱法/高效液相/卡波姆Key words
Acne gel/ Hollow fiber/ Content determination/ HPLC/ Carbomer分类
医药卫生引用本文复制引用
马亚中,吴燕,刘丽平,林向阳..中空纤维分离-高效液相色谱法测定复方痤疮凝胶中主药的含量[J].医药导报,2011,30(10):1340-1343,4.
