工业微生物2011，Vol.41Issue(4)：1-5,5.DOI:10.3969/j.issn.1001-6678.2011.04.001
游动放线菌中orf20基因敲除对雷莫拉宁合成的影响
Effects of gene disruption of orf20 on ramoplanin production in Actinoplanes sp.
摘要
Abstract
The orf20 in the ramoplanin biosynthesis gene cluster shows high homology to the known halogenases . An inframe knock-out plasmid pSM-3 was transformed into Actinoplanessp . ATCC 33076 to delete the orf20 gene fragm entencoding 64 am inoacids. A representative orf20 mutant was designated SIPI-A .2001 dorf20 (△orf20 ) .Absence of ramoplanin and its analogs production from the rsulting exconjugants suggested that the protein coded by orf20 was a key enzyme in the ram oplanin biosynthesis pathway , and it might be have other funcions except halogenation .关键词
雷莫拉宁/基因敲除/同源重组/卤化酶Key words
ramoplanin / gene knockout/ hom ologous recom bination / halogenase引用本文复制引用
苏敏,邵雷,刘爱娟,林慧敏,陈代杰,李继安..游动放线菌中orf20基因敲除对雷莫拉宁合成的影响[J].工业微生物,2011,41(4):1-5,5.基金项目
"重大新药创制"科技重大专项(No.2009ZX09301-007) （No.2009ZX09301-007）
国家科技支撑计划(No.2008BAI63B01) （No.2008BAI63B01）
国家自然科学基金(No.30772678) （No.30772678）
