华西口腔医学杂志2011，Vol.29Issue(4)：413-414,2.DOI:10.3969/j.issn.1000-1182.2011.04.020
金属栅栏式腭器官培养模型的建立
Establishment of palatal organ culture in vitro
摘要
Abstract
Objective The purpose of this study was to establish a palatal organ culture method and to investigate the palatogenesis in vitro. Methods 20 pregnant 14-day mice were killed, embryos were separated ascetically, and palatal shelves were dissected and placed on a modified Trowell's system. All explants were cultured 24 h and 48 h respectively. Finally, all explants were embedded and stained by Hematoxylin and Eosin. Results All explants grew healthy. After incubation for 24 h, medial edge epithelium maintained, whereas after 48 h, medial edge epithelium disappeared, bilateral mesenchymal cells contacted, palates fused. Conclusion This method provides an effective way for investigating the etiology of cleft palate in vitro.关键词
腭器官/器官培养/腭裂Key words
palatal organ/ organ culture/ cleft palate分类
医药卫生引用本文复制引用
卢胜军,何苇,石冰,蒙田,李承浩,封兴华..金属栅栏式腭器官培养模型的建立[J].华西口腔医学杂志,2011,29(4):413-414,2.基金项目
国家自然科学基金重点资助项目(2006-C030304) （2006-C030304）
