华西口腔医学杂志2011，Vol.29Issue(4)：415-419,5.DOI:10.3969/j.issn.1000-1182.2011.04.021
脂多糖和白细胞介素-1β对人牙周膜细胞中诱导型一氧化氮合酶表达的影响
Effect of lipopolysaccharide and interleukin-1β on the expression of inducible nitric oxide synthase gene in human periodontal ligament cells
摘要
Abstract
Objective To observe the effect of lipopolysaccharide(LPS) and interleukin-lβ(IL-1β) on the expression of inducible nitric oxide synthase (iNOS) gene and nitric oxide (NO) in human periodontal ligament cells (hPDLCs).Methods After stimulating hPDLCs by LPS and IL-1β, RT-PCR had been used to identify the expression of iNOS gene.The activity of iNOS in the culture was quantitated by enzyme-linked immune sorbent assay (ELISA).And the level of NO was determined by nitrate reductase method.Results Slight amount of iNOS and NO had been detected in hPDLCs without sitimulation, but when stimulating with LPS and IL-1β, the amount of the iNOS mRNA increased significantly in the cells in the time and dose dependent way (P＜0.05).Under the stimulation with the same time or same dose, the productions of iNOS and NO stimulated by IL-1β and in combination with LPS were larger than that stimulated with LPS(P＜O.O5).Conclusion The expression NO and iNOS could be increased by stimulating hPDLCs with LPS and IL-1β, which may contribute to the research on injecting LPS and IL-1β at periodontal tissue of animal models.关键词
人牙周膜细胞/脂多糖/白细胞介素-1β/诱导型一氧化氮合酶Key words
human periodontal ligament cells/ lipopelysaccharide/ interleukin-1β/ inducible nitric oxide synthase分类
医药卫生引用本文复制引用
徐婧秋,吴丽萍..脂多糖和白细胞介素-1β对人牙周膜细胞中诱导型一氧化氮合酶表达的影响[J].华西口腔医学杂志,2011,29(4):415-419,5.基金项目
国家自然科学基金资助项目(C03031104) （C03031104）
