中国药理学通报2011，Vol.27Issue(10)：1430-1434,5.DOI:10.3969/j.issn.1001-1978.2011.10.023
黄芪甲苷对慢性哮喘模型小鼠气道重塑的影响
Effects of Astragaloside Ⅳ on airway remodeling in a murine model of chronic asthma
摘要
Abstract
Aim To observe the effects of Astragaloside lV on the airway remodeling in a murine model of chronic asthma. Methods 48 BALB/c mice were randomly divided into 4 groups , namely Control group ,Asthma group( OVA group ), Astragaloside Ⅳ group and Budesonide group with 12 mice in each group.BALB/c mice sensitized to ovalbumin ( OVA ) were chronically challenged with aerosolized OVA for eight weeks. Mice in the Astragaloside Ⅳ group were intragastrically administered with Astragaloside Ⅳ ( 50 mg · kg-1 ) daily for 8 consecutive weeks. Mice in the Budesonide group were exposed to an aerosol of budesonide ( 8 ml ) daily for 8 consecutive weeks. 24 hours after the last OVA challenge , pulmonary functions were measured to evaluate the resistance of expiration in 6 mice of each group. Cells in BAL fluid ( BALF ) were counted. The sections were stained with either hematoxylin & eosin to assess the inflammatory cell infiltrates. and Masson's trichrome to determine subepithelial fibrosis in the lungs. The levels of VEGF in BALF were measured by ELISA. The expression of α-SMA and VEGF protein in lungs was detected by immunohistochemistry. Results Treatment with Astragaloside lV markedly inhibited the airway inflammation, airway hyperresponsiveness ( AHR ) and remodeling. Astragaloside Ⅳ significantly decreased the level of VEGF in BLAF. In addition,Astragaloside Ⅳ obviously attenuated the protein expression of α-SMA and VEGF in lungs. Conclusions Astragaloside lV can suppress the progression of airway remodeling in a murine model of chronic asthma. Partly, the effects might be due to inhibition of the expression of VEGF.关键词
黄芪甲苷/哮喘/小鼠/气道重塑/血管内皮生长因子/α-平滑肌肌动蛋白Key words
Astragaloside Ⅳ / asthma/ mice/ airway remodeling/ VEGF/α-SMA分类
医药卫生引用本文复制引用
杜强,张倩,沈立,蔡健康,黄茂,殷凯生..黄芪甲苷对慢性哮喘模型小鼠气道重塑的影响[J].中国药理学通报,2011,27(10):1430-1434,5.基金项目
南京医科大学校基金重点资助项目(No 09NMUZ23) （No 09NMUZ23）
