中国药理学通报2011，Vol.27Issue(10)：1439-1443,5.DOI:10.3969/j.issn.1001-1978.2011.10.025
预先给予酪氨酸激酶抑制剂IPTRK3对神经源性疼痛的影响
Effects of TrkA inhibitor IPTRK3 pretreatment on neuropathic pain
摘要
Abstract
Aim To investigate the inhibitory effects of pretreatment TrkA inhibitor IPTRK3 on neuropathic pain induced by partial sciatic nerve ligation ( PSNL )and its possible mechanisms. Methods Partial sciatic nerve ligation models were established in mice. All drugs were injected intraperitoneally 10 min before surgery. Paw Withdrawal Latency( PWL )and 50％ Paw Withdrawal Threshold ( PWT ) were measured at different time points pre or post drug administration. Dorsal root ganglions( DRG ) of Left L4-5 were removed for determination of TRPVI protein expression with Western blot, and L4-5 spinal dorsal horn tissues were removed for analyzing the expression of Fos protein by immunohistochemistry. Results Compared with sham group 2 h after surgery, mice in PSNL group developed a robust thermal and mechanical hyperalgesia and TRPVI in DRG and Fos protein expressions in spinal dorsal horn were increased; compared with PSNL group, TrkA inhibitor IPTRK3 obviously attenuated thermal hyperalgesia and significantly suppressed the expression of TRPVI in DRG and Fos protein in spinal dorsal horn. Conclusion Pre-emptive administration of IPTRK3 obviously inhibits the up-regulation of TRPVI in DRG and Fos protein in spinal dorsal horn, which may be possibly associated with its rapid anti-hyperalgesia effects in PSNL model of neuropathic pain.关键词
TrkA/IPTRK3/坐骨神经部分结扎/神经源性疼痛/TRPV1/Fos蛋白Key words
TrkA / IPTRK3 / partial sciatic nerve ligation/ neuropathic pain / TRPVI / Fos protein.分类
医药卫生引用本文复制引用
马伟英,何惠燕,纪风涛,刘玲,梁建军,广濑宗孝,曹铭辉..预先给予酪氨酸激酶抑制剂IPTRK3对神经源性疼痛的影响[J].中国药理学通报,2011,27(10):1439-1443,5.基金项目
广东省科技计划资助项目(No 2010B080701004) （No 2010B080701004）
广东省医学科研基金资助项目(No B2010092) （No B2010092）
