中国药理学通报2011，Vol.27Issue(10)：1448-1451,4.DOI:10.3969/j.issn.1001-1978.2011.10.027
血管紧张素Ⅱ1型受体拮抗剂用于脓毒症大鼠的实验研究
Anti-inflammatory effect of losartan on LPS-induced sepsis in rats
摘要
Abstract
Aim The purpose of this study was to investigate role of angiotensin Ⅱ 1 receptor ( ATIR ) antagonist on LPS-induced sepsis in rats.Methods Thirty male SD rats were randomly divided into 3groups ( n = 10 each ) , rats were injected with 9 mg ·ml-1 NaCl solution ( Control group ), or received LPS 12 mg · kg-1 intravenously ( LPS group ), or accepted infusion of an AT1 receptor antagonist , losartan 50 mg · kg-1 intraperitoneally 30 min before LPS 12 mg ·kg -1 was intravenously administered( LOS group ) .The plasma concentrations of nitric oxide ( NO ), malondialdehyde ( MDA ),IL-1β and TNF-α were measured in all rats 6 h after LPS administration, then all the rats were killed immediately before the aortas were removed and the inhibitor of NF-κB ( IKB ) mRNA and protein expressions in aortas were determined.Results The plasma concentrations of NO, MDA , IL-1β and TNF-α were all significantly elevated in LPS group compared with the control group ( P＜ 0.01 ), which were markedly reduced in LOS group ( P ＜ 0.01 ).Both of the IκB mRNA and protein expressions in aortas were down-regulated after injection of LPS compared with the control group( P ＜ 0.01 ).However, in the LOS group,IκB mRNA and protein expressions in aortas were obviously higher than the LPS group( P ＜ 0.01 ).Conclusion AT1 receptor antagonist losartan exerts anti-inflammatory effects on sepsis rats.关键词
脓毒症/血管紧张素Ⅱ1型受体/拮抗剂/内毒素/氯沙坦/IκBKey words
sepsis/ angiotensin Ⅱ 1 receptor antagonist / antagonist / endotoxin / losartan / IκB分类
医药卫生引用本文复制引用
廖丽君,郭建荣,贾东林,喻君,沈华春..血管紧张素Ⅱ1型受体拮抗剂用于脓毒症大鼠的实验研究[J].中国药理学通报,2011,27(10):1448-1451,4.基金项目
宁波市医学科技基金资助项目(No 200504) （No 200504）
