医药导报2011，Vol.30Issue(10)：1269-1272,4.DOI:10.3870/yydb.2011.10.004
高效液相色谱-质谱串联法测定人血浆特拉唑嗪的浓度
HPLC-MS/MS Determination of Terazosin in Human Plasma
侯允天 1郝光涛 2郑专杰 2梁海霞 3马蒙 4朱力军 4刘泽源2
作者信息
- 1. 解放军总医院老年心血管病研究所,北京,100853
- 2. 军事医学科学院附属医院临床药理室,北京,100071
- 3. 首都医科大学附属北京安定医院药剂科,100088
- 4. 蚌埠医学院药学系,安徽蚌埠,233030
- 折叠
摘要
Abstract
Objective To establish a simple and sensitive high performance liquid chromatography tandem mass spectrometry ( HPLC-MS/MS ) method for determination of terazosin in human plasma. Methods The plasma samples were precipitated with the liquid extraction by tert-butyl ether and then were injected directly into the HPLC - MS/MS system. Inertsil C18 column ( 2. 1 mm×150 mm,3.0 μm ) was used, and methanol- 2 mmol · L-1 ammonium acetate ( 90 ∶ 10 ) was taken as the mobile phase by gradient elution. Terazosin and the internal standard ( prazosin ) were separated by HPLC and quantitated by MS/MS by using electrospray ionization ( ESI ) and multiple reaction monitoring ( MRM ) in the positive ion mode. The most intense [ M-H ]+ MRM transition of terazosin at m/z 388.4 →m/z 247. 4 was used for quantitation, and the transition at m/z 384. 2 →m/z 231. 3 was used to monitor prazosin. Results The liner range of terazosin was 0. 2-50 ng · mL-1( r= 0. 9973 ), while the minimal detectable plasma concentration was 0. 2 ng · mL-1 . The average recovery was between 98. 83％ and 100. 67％ . Intra-day and inter-day RSD were less than 15％ . Low, medium and high absolute recoveries were 75. 40％ , 70. 28％ , 67. 39％ , in line with biological sample analysis requirements. Conclusion This method is simple , sensitive and accurate, which is suitable for the study of pharmacokinetics of terazosin in human plasma and urine.关键词
特拉唑嗪/血药浓度/高效液相色谱-质谱串联法Key words
Terazosin/ Plasma concentration/ HPLC-MS/MS分类
医药卫生引用本文复制引用
侯允天,郝光涛,郑专杰,梁海霞,马蒙,朱力军,刘泽源..高效液相色谱-质谱串联法测定人血浆特拉唑嗪的浓度[J].医药导报,2011,30(10):1269-1272,4.
