摘要
Abstract
Objective To observe the effects of breviscapine on the expression of TGF - betal and Smad7 in myocardium of diabetic rats. Methods Out of 30 health SD rats , 20 were used for establishment of diabetic rat model and subsequently assigned into DM group and breviscapine treatment group ( Bre group ). The other 10 rats were used as controls. Intraperitoneal injection of breviscapine parenteral solution [ 20 mg/( kg · d ) ] was performed in bre group, while saline substitution was applied in DM and control group. Caudal venous blood glucose measurement, cardiac function,body weight ( BW ), heart weight ( HW ) and HW/BW were assessed in 12th week. The ultrastructural and myocardial structure pathological changes were evaluated by electron microscopy and light microscopy, respectively. The protein levels of TGF - betal and Smad7 were detected hy immunohistochemistry. Results There was no significant difference in blood sugar between Bre and DM group ( P ＞ 0. 05 ). Reduction of cardiac index, and improvement of maximum velocities of rise and decline of left ventricular pressure were recorded in Bre - treated diabetic rats ( P ＜ 0. 05 ). Meanwhile, significant alleviation of myocardial pathological changes, down - regulation of myocardial TGF - betal , and up - regulation of Smad7 were also observed in Bre - treated diabetic rats ( P ＜ 0. 05 ). Conclusion Bre viscapine alleviates myocardial pathological changes and improves cardiac function in diabetic rats, via down - regulation of myocardial TGF - beta1 and up - regulation of Smad7 , thus inhibits diabetic myocardial fibrosis.关键词
灯盏花素/糖尿病心肌病变/转化生长因子-β1/SMAD7蛋白Key words
breviscapine/ diabetic cardiomyopathy/ TGF - beta1/ Smad7
