华西口腔医学杂志2011，Vol.29Issue(4)：355-357,3.DOI:10.3969/j.issn.1000-1182.2011.04.005
口腔链球菌密度感应信号系统comE基因及luxS基因的检测分析
Detection and analysis of comE and luxS genes in quorum sensing signal pathway from
徐蓉蓉 1王斌 2葛久禹1
作者信息
- 1. 南京大学口腔医学院,口腔内科学教研室,南京,210008
- 2. 南京大学生命科学院,分子进化实验室,南京,210093
- 折叠
摘要
Abstract
Objective To detect and analyze two important genes, comE and luxS, in quorum sensing signal pathway from Streptococcus oralis (S.oralis).Methods The total genomic DNA of S.oralis NH521 (a clinically isolated strain)was firstly extracted.The comE and luxS genes were then amplified by polymerase chain reaction(PCR) and further sequenced.The obtained sequences were compared with related sequences in GenBank.Results Target bands of both comE and luxS genes were detected by electrophoresis.The obtained gene sequences were similar to the corresponding sequences from another S.oralis strain(luxS, 95.0％; comE, 99.6％); however, comparing to gene sequences of another species Streptococcus mutans, comE was more divergent(12.7％) than luxS gene(74.1％).Conclusion This study successfully amplified and sequenced comE and luxS genes from S.oralis NH521 strain.The luxS gene accumulated more mutations than comE gene did between two S.oralis strains, but comE gene is more divergent than luxS gene between two Streptococcus species.关键词
口腔链球菌/密度感应/comE基因/luxS基因Key words
Streptococcus oralis/ quorum sensing/ comE gene/ luxS gene分类
医药卫生引用本文复制引用
徐蓉蓉,王斌,葛久禹..口腔链球菌密度感应信号系统comE基因及luxS基因的检测分析[J].华西口腔医学杂志,2011,29(4):355-357,3.
