工业微生物2011，Vol.41Issue(4)：61-66,6.DOI:10.3969/j.issn.1001-6678.2011.04.012
产耐热β-葡萄糖苷酶细菌的筛选与鉴定
Isolation and identification of bacteria for producing thermophilic β-glucosidase
廖德芳 1陈献忠 1张梁 1王正祥 1石贵阳1
作者信息
- 1. 江南大学生物资源与能源研究中心,无锡,214122
- 折叠
摘要
Abstract
From 1323 bacteria strains collected by the Culture Collection Center of Industrial Microorganism sin Jiangnan Uninversity (CIC IM-CU ), 20 strains with relatively high capability of producing therm ophilic β-glucosidase were isolated . 5 strains were identified as Geobacillus sp . and the others were identified as B acillus licheniform is by 16s rDN A sequencing analysis . C rude enzym es was characterized from Geobacillus sp . (170-28-2 ) and B acinus licheniform is (F157043A ) . The Geobacillussp . with a specific activity of 0.36 U/m g showed optimal activity at pH 8 .0 and 53℃ and the B acillus licheniform is with a specific activity of 0.66 U /m g show ed optim alactivity at pH 9 .0 and 55℃ . Based on morpholoigcal, physiological and biochem ical properties , the strain of F157043A was further identfied as Bacillus lichen iform is .关键词
耐热β-葡萄糖苷酶/细菌/筛选/鉴定Key words
therm ophilic β-glucosidase / bacteria/ screening / identfication引用本文复制引用
廖德芳,陈献忠,张梁,王正祥,石贵阳..产耐热β-葡萄糖苷酶细菌的筛选与鉴定[J].工业微生物,2011,41(4):61-66,6.
