工业微生物2011，Vol.41Issue(4)：67-71,5.DOI:10.3969/j.issn.1001-6678.2011.04.013
紫外线诱变选育聚丁二酸丁二醇酯(PBS)高降解活性菌株
Screening of poly(butylenes succinate)(PBS) high-degrading activity strain by UV mutagenesis
摘要
Abstract
By U V mutation , clear-zone method for initial screeing and test of degrading PBS film for re-screening , a m utant strain H J1O with higher ability of degrading PBS film and higher endurance to the temperature and pH valie was obtained from the original strain Alternaria sp . H J03 . Compared with the origmal strain , H J10 had less aenalm ycelium at the begmning of culture , but after 7 days the colony turned com pact and the growth rate was faster . In addition , the herEditary character of higher degrading ability of the mutant was stable after 7 generations, and its degradation rate increased over 14.4％ . The degrading ability of the mutant strain H Jl0 was higher than that of the initial strain at the optimum tem perature (25℃～35℃ ) , even at inappropriate tem perature . Besides, the degradation rate of H Jl0 was also improved at different pH values , especially when the pH value was 5.0 , w hich lincreased by 22 .80％ .关键词
紫外线诱变/突变菌株/聚丁二酸丁二醇酯(PBS)/降解性Key words
U V mubgenesis / mutant strain / poly (butylenes succinate ) (PBS ) / degradation引用本文复制引用
董骞,梁英梅,田呈明,孙琪,梅雪立..紫外线诱变选育聚丁二酸丁二醇酯(PBS)高降解活性菌株[J].工业微生物,2011,41(4):67-71,5.基金项目
教育部留学回国基金和科技部林业微生物菌种保藏平台项目(No.2005DKA21207-8). （No.2005DKA21207-8）
