安徽农业科学2011，Vol.39Issue(17)：10121-10123,3.
小麦SRAP-PCR体系的正交设计优化
Optimization of SRAP-PCR System of Triticum aestivum-Based on Orthogonal Design
摘要
Abstract
[ Objective ] The aim was to optimize the technical procedure of SRAP-PCR in Triticum aestivum L.. [ Method ] The orthogonal design was used to optimize SRAP-PCR amplification system of wheat Fengyou 68 on five factors ( Taq polymerase, Mg2+ , DNA template,dNTPs and primer) at four levels. [ Result ] Effects of the five factors on SRAP-PCR reaction system were Mg2 + ＞ Taq polymerase ＞ dNTPs ＞DNA template ＞ primer. Finally, an optimal SRAP-PCR system was established, that was the total 20 μl reaction system containing 2.0 μl 10 × PCR Buffer, 2.0 U DNA polymerase, 0. 2 mmol/L dNTPs, 40 ng DNA template and 0. 6 μmol/L primer. [ Conclusion] The optimum SRAP-PCR system had provided some technical foundations to study the phylogenetic relationship of wheat varieties.关键词
小麦/SRAP/正交设计/优化Key words
Wheat/SRAP/Orthogonal design/Optimization分类
农业科技引用本文复制引用
艾鹏飞,武玉芬,魏景芳..小麦SRAP-PCR体系的正交设计优化[J].安徽农业科学,2011,39(17):10121-10123,3.基金项目
国家科技支撑计划项目(2008 FY110500-9). （2008 FY110500-9）
