安徽农业科学2011，Vol.39Issue(17)：10124-10126,10129,4.
白假丝酵母脂肪酶CaLIP5在毕赤酵母X33中的优化重组表达
Optimum Production of Recombinant Candida albicans Lipase 5 Expressed in Pichia pastoris X33
摘要
Abstract
[ Objective ] To develop new lipases and optimize the fermentation medium of lipase. [ Method ] The lip5 gene of C. albicans was first cloned and heterogenously expressed in Pichia pastoris X33 system. Response surface methodology was adopted to construct the regression equation for the lipolytic activity of CaLIP5. [ Result] Under 25.71 ℃, pH 8.33, 1.28％ yeast extract, and 4.06％ glucose concentration,the predicted maximum lipolytic activity was 8.33 U/ml, and the actual value was 9.24 U/ml. The actual value and the predicted value were basically the same. The optimized lipolytic activity was 22.22％ higher than before. [ Conclusion ] Highest lipolytic activity of recombinant CaL1P5 was obtained in the optimized fermentation progress.关键词
白假丝酵母脂肪酶/重组表达/优化培养基/响应面法Key words
Lipase of Candida albicans/Heterogeneous expression /Optimization of medium /Response surface methodology分类
农业科技引用本文复制引用
杨宁,蓝东明,王永华,杨博..白假丝酵母脂肪酶CaLIP5在毕赤酵母X33中的优化重组表达[J].安徽农业科学,2011,39(17):10124-10126,10129,4.基金项目
广州市科技攻关项目(2009A1-E). （2009A1-E）
