安徽农业科学2011，Vol.39Issue(17)：10143-10146,4.
以pmi为筛选标记的水稻转基因研究
Development of Rice GeneticTransformation Using pmi Gene as A Selectable Maker
摘要
Abstract
[ Objective ] The research aimed to establish a rapid, effective and safety mannose screening system. [ Method ] The phosphomannose isomerase gene (pmi) was transformed into Japonica rice variety Wanjing 97 with green fluorescent protein (GFP) gene as rcporter gene. Further, the transformation and selection conditions were optimized. [ Result] The results showed that callus emerging rate could be up to 90.3％ and the growth rate under continuous illumination at 32 ℃ was much higher than the other conditions. The pressure of 5 g/L sucrose plus + 15 g/L mannose was the best condition for selective medium. Under this optimal condition, the transgenic plants could be generated after 21 d, and the transformation efficiency could up to 20.6％. [ Conclusion] The study developed a short-time consuming selection system with high transformation efficiency which facilitates to improve the safety of transgenic rice production.关键词
水稻/转基因/甘露糖/绿色荧光蛋白(GFP)Key words
Rice/ Transgene/ Mannose/ Green fluorescent protein (GFP)分类
农业科技引用本文复制引用
梁永亚,李浩,段永波,陆徐忠,唐甜甜,杨剑波,李莉..以pmi为筛选标记的水稻转基因研究[J].安徽农业科学,2011,39(17):10143-10146,4.基金项目
科技部转基因重大专项(2009ZX08001-013B) （2009ZX08001-013B）
科技部国际合作项目(2008DFA31840). （2008DFA31840）
