安徽农业科学2011，Vol.39Issue(17)：10147-10149,10184,4.
抗松针褐斑病湿地松子代组培苗瓶内菌根化研究
Study on the Mycorrhizal Formation in the Offspring Tissue Culture Bottle of Pinus elliottii with the Brown Spot Needle Blight Resistance
摘要
Abstract
[ Objective ] The research aimed to study the mycorrhizal formation in the offspring tissue culture bottle of Pinus elliottii with the brown spot needle blight resistance. [ Method ] The mycorrhizal formation under the vitro condition of Pinus elliottii regenerated plant was studied, and the growth condition of regenerated plant was observed. [ Result ] The mycorrhiza fungi culture substrate and the inoculation amount had the big influence on the mycorrhizal formation under the vitro condition. After the primordial rooting was induced from Pinus elliottii buds,it was best to be transplanted into the perlite medium and inoculate Pisolithus tinctorius, which was favorable for the mycorrhizal formation.When the two blocks of Pisolithus tinctorius were inoculated in the perlite medium, the mycorrhizal formation rate reached 84.4％. The dichotomous branching short root was the most, and 12.49 roots were formed on every main root. The mycorrhizal formation improved the survival rate of regenerated plant domestication transplant. The mycorrhizal formation regenerated plant grew well in the phytotron, and the root system was developed. [ Conclusion] The research provided the basis for improving the survival rate of Pinus elliottii tissue culture regenerated plant with the brown spot needle blight resistance.关键词
抗性湿地松/再生植株/彩色豆马勃/菌根化Key words
Resistant Pinus elliottii/ Regenerated plant/ Pisolithus tinctorius/ Mycorrhizal formation分类
农业科技引用本文复制引用
安会翠,叶建仁,吴小芹,刘戈,朱丽华..抗松针褐斑病湿地松子代组培苗瓶内菌根化研究[J].安徽农业科学,2011,39(17):10147-10149,10184,4.基金项目
国家林业局"948"项目(2011-4-69). （2011-4-69）
