安徽农业科学2011，Vol.39Issue(17)：10150-10152,3.
Taqman定量PCR技术检测转基因大豆中外源基因拷贝数
Detection of Foreign Gene Copies in Transgenic Soybean by Taqman Quantitative PCR Technique
摘要
Abstract
[ Objective ] It is to adopt Taqman quantitative PCR technique to detect the copies of foreign nos terminator in transgenic hybrid soybean. [ Method ] With endogenous reference gene of soybean lectin, and endogenous reference standard of gene complex DNA in non-GMO soybeans, the method of gradient dilution was used to separately calculate Ct value of endogenous reference gene and plasmid DNA and relevance standard curve equation of logarithm of copies, and then to calculate the copies of samples through substituting thus-obtained Ct into standard curve equation. [ Result ] The standard curve equation of endogenous reference gene isy = - 3.422x + 35. 201 , R2 = 0. 998; and the standard curve equation of foreign gene is y = - 3. 348x + 34. 890, R2 = 0.999. Nos terminator and its lower boundary sequences in transgenic soybean is of single copy. [ Conclusion] The study has provided a theoretical basis for determining foreign gene copies in transgenic soybean.关键词
Real-time PCR/转基因杂交大豆/拷贝数/Lectin/nos终止子基因边界序列Key words
Real-time PCR/Transgenic hybrid soybean/Copies/Lectin/Boundary sequence of nos terminator gene分类
农业科技引用本文复制引用
仇有文,张明辉,高学军,曲波,敖金霞,袁肖寒,刘营,霍楠..Taqman定量PCR技术检测转基因大豆中外源基因拷贝数[J].安徽农业科学,2011,39(17):10150-10152,3.基金项目
哈尔滨市科技局项目(2010RFQXNI01) （2010RFQXNI01）
转基因重大专项课题子课题(2008ZX08012-001). （2008ZX08012-001）
