临床与实验病理学杂志2011，Vol.27Issue(10)：1048-1051,4.
胃癌hMSH2基因突变与HP感染的关系
Correlation between mutation of gene hMSH2 and HP infection in gastric carcinoma by laser capture microdissection
摘要
Abstract
Purpose To study the correlation hetween mutation of gene hMSH2 and HP infection in gastric carcinoma, and to evaluate their clinicapathological characteristics and the possible mechanism of carcinogenesis caused by HP infection.Methods ( 1 ) 45 cases of gastric carcinoma were examined.HP infection was examined by using Warthin-Starry method.( 2 ) The cells of gastric carcinoma were got through laser capture microdissection.( 3 ) 45 cases of gastric carcinomas were studied respectively with five exons of hMSH2 gene mutation hy using polymerase chain reaction( PCR ), single strand conformation polymorphism( SSCP ) and silver-staining.Results ( 1 ) The negative rate of HP infection in 45 cases of gastric carcinoma was 42.20％ and the positive rate was 57.78％.( 2 ) In 45 cases of gastric carcinoma, five exons mutation rate of hMSH2 gene was 8.89％ and there was no significantly associated with gender, age , location, lymph node metastases and histologic type, differentiation and stage of clinical pathology( P ＞ 0.05 ).( 3 ) The hMSH2 gene mutation rates of the gastric carcinoma with HP infection and without HP infection were 15.38％ and 0 respectively.There were no significant difference between them( P ＞ 0.05 ).Conclusions HP infection maybe not related to hMSH2 gene mutation in gastric carcinoma.关键词
胃肿瘤/激光捕获显微切割/hMSH2/基因突变/幽门螺旋杆菌Key words
stomach neoplasms/ laser capture microdissection/ hMSH2 / mutation/ helicobacter pylori分类
医药卫生引用本文复制引用
刘连,王丽珍,李春鸣..胃癌hMSH2基因突变与HP感染的关系[J].临床与实验病理学杂志,2011,27(10):1048-1051,4.基金项目
贵州省优秀青年科技人才培养计划[黔科合人字(2005)0512号] （2005）
