肿瘤防治研究2011，Vol.38Issue(8)：881-885,5.DOI:10.3971/j.issn.1000-8578.2011.08.008
威灵仙皂苷对急性早幼粒细胞白血病细胞株NB4细胞的凋亡诱导作用及其机制
Radix Clematidis Saponins Inhibits NB4 Cells and through Inducement of Apoptosis
摘要
Abstract
Objective To investigate the effect of Radix Clematidis Saponins(RCS) on NB4 cells apoptosis and its possible mechanism. Methods NB4 cells were treated with different concentrations of Radix Clematidis Saponins, using 0. 01 ％ DMSO as the negative control group, 1.0 μmol/L Arsenic Trioxide as the positive control group. MTT method was used to detect the inhibition rate. The apoptostic rate and the alteration of cell cycles were assessed by flow cytometry determination and cell morphology was observed by wright's staining. Real-time PCR was used to evaluate the effects of sRadix Clematidis Saponins on the PML-RARa mRNA. Results Radix Clematidis Saponins was able to inhabit the NB4 cell growth, in time or concentration-dependent manner, but the inhibition rate was less than Arsenic Trioxide group. Wright's staining showed typical apoptotic morphological changes in Radix Clematidis Saponins group, the same as Arsenic Trioxide group. Apoptotic rate was increased in time dependent manner. The number of G2 stage cells was increased, while G1 stage was decreased (P＜0. 05). There was no difference of expression of PML-RARa mRNA between two groups or between different time periods of Radix Clematidis Saponins treatment. Conclusion Radix Clematidis Saponins might induce cell apoptosis, possibly by cell cycle arresting, but not reduce the PML-RARa mRNA gene expression.关键词
威灵仙皂苷/急性早幼粒细胞性白血病/NB4细胞/细胞凋亡Key words
Radix Clematidis Saponins/ Acute promyelocytic leukemia/ NB4 cell/ Apoptosis分类
医药卫生引用本文复制引用
周云,黄纯兰,李录克,李晓明..威灵仙皂苷对急性早幼粒细胞白血病细胞株NB4细胞的凋亡诱导作用及其机制[J].肿瘤防治研究,2011,38(8):881-885,5.基金项目
四川省卫生厅基金资助项目(07023) （07023）
泸州市科技局基金资助项目(07073) （07073）
泸州医学院附属医院人才基金资助项目(07043) （07043）
