肿瘤防治研究2011，Vol.38Issue(8)：909-913,5.DOI:10.3971/j.issn.1000-8578.2011.08.014
甲状腺癌NIS和TSHR表达的矛盾性及非相关性
Contradiction and Non-correlation between NIS and TSHR Expression in Human Thyroid Cancer
王志峰 1刘勤江 2廖世奇 3葛廷 3杨荣4
作者信息
- 1. 730000,兰州,兰州大学生命科学院细胞所
- 2. 甘肃省肿瘤医院头颈科
- 3. 甘肃省医学科学研究院分子生物学中心
- 4. 甘肃省肿瘤医院病理科
- 折叠
摘要
Abstract
Objective To investigate the correlation between sodium/iodide symporter and thyroid stimulating hormone receptor expression in human thyroid cancer.Methods mRNA levels, quantified by realtime PCR, and protein evaluated by immunohistochemistry , were examined in surgical specimens of 18 normal thyroid tissues and 23 thyroid carcinoma tissues.Results The NIS and TSHR mRNA levels of thyroid carcinoma were significantly lower than those of normal thyroid tissue and nodular goiter(P＜0.005).In addition , there was a significant positive correlation between NIS and TSHR in normal thyroid samples ( r = 0.667, P = 0.000), but no such correlation in thyroid carcinomas ( r = 0.222, P =0.376).The immunohistochemistry results showed that the NIS protein mainly located in cytoplasm,and TSHR protein mainly located in cytoplasm and cytomembrane.In thyroid carcinomas , the positive rates of NIS and TSHR protein were markedly higher than those in normal thyroid tissues(P＜0.05).Through combined analysis of NIS mRNA concentrations and immunostaining scores, in the thyroid carcinomas, reduction of NIS mRNA expression with increment of intracellular protein was found in 74％ (17/23) of the samples compared with normal tissues.Conclusion In thyroid carcinomas ,there were expressed contradiction and non-correlation between NIS and TSHR expression.NIS was not able to play the role of protein transporting and absorbing iodine as mainly located in cytoplasm, which was regarded as an important reason of radioiodine therapy failure for thyroid carcinoma.关键词
甲状腺癌/钠/碘转运蛋白/促甲状腺激素受体/荧光定量分析/免疫组织化学Key words
Thyroid carcinoma/ Sodium/iodide symporter/ Thyroid stimulating hormone receptor/ Quantitative fluorescence analysis/ Immunohistochemistry分类
医药卫生引用本文复制引用
王志峰,刘勤江,廖世奇,葛廷,杨荣..甲状腺癌NIS和TSHR表达的矛盾性及非相关性[J].肿瘤防治研究,2011,38(8):909-913,5.
