安徽农业科学2011，Vol.39Issue(17)：10116-10118,3.
外源抗菌肽在转基因鱼腥草中的表达及抗茎腐病研究
Expression of Exogenous Anfimicrobial Peptide and Resistance to Stem Rot Disease of Houttuynia cordata Thunb.
摘要
Abstract
[ Objective ] This study was to identify the expression of exogenous antimicrobial peptide in transgenic Houttuynia cordata Thunb.plants, and analyze their resistance to stem rot disease. [ Method] SDS-PAGE and Western blot analysis were employed to detect expression of exogenous antimicrobial peptide in transgenic H. cordata plants. Both wild type and transgenic H. cordata plants were inoculated with different concentrations of Rhizoctonia solani spores for detecting their resistance. [ Result ] The exogenous antimicrobial peptide was detected at translation level. The optimal parameters for detecting the resistance of transgenic H. cordata plants to R. solani was inoculation of spores at a concentration of 3 x 105 ind./ml and cultured for three days. The results showed that resistance of transgenic H. cordata plants to R. solani was enhanced in comparison with CKs. [ Conclusion] Expression of exogenous antimicrobial peptide can enhance the resistance of transgenic H.cordata plants to stem rot disease.关键词
转基因鱼腥草/抗菌肽/抗病性Key words
Transgenic Houttuynia cordata Thunb. /Antimicrobial peptide/ Disease resistance分类
农业科技引用本文复制引用
易浪,董燕,周联,刁玲武,朱丹丹..外源抗菌肽在转基因鱼腥草中的表达及抗茎腐病研究[J].安徽农业科学,2011,39(17):10116-10118,3.基金项目
国家自然科学基金项目(30772737). （30772737）
