安徽农业科学2011，Vol.39Issue(17)：10127-10129,3.
巴西橡胶树树根组织培养外植体消毒研究
Studies on the Explant Disinfection in the Root Tissue Culture of Hevea brasiliensis
摘要
Abstract
[ Objective ] The aim was to look for an effective way of decreasing explant contamination in the root tissue culture of Hevea brasiliensis. [ Method]The root tissue culture of H. brasiliensis was carired out taking news roots after treatment for 30 d as explants,and explants was disinfected by using 1 g/L carbendazim,0. 1％ HgCl2 and Yipeilong with different concentration. [ Result] Explants were treated by 1 g/L carbendazim before conventional disinfection and disinfected by 75％ ethyl for 30 s and 0.1％ HgCl2 for 6 min,successively, finally explants were inoculated in solid callus induction medium with 0. 1％ Yipeilong and cultured for 30 d when the contamination rate of explants decreased to 44.59％ and its survival rate was up to 25.60％. [ Conclusion] The study could provide theoretical foundation for the decrease of explant contamination in the root tissue culture of H. brasiliensis.关键词
巴西橡胶树/树根外植体/污染控制/多菌灵/益培隆Key words
Hevea brasiliensis / Root explants/Contamination control/Carbendazim/Yipeilong分类
农业科技引用本文复制引用
姜泽海,周权男,李哲..巴西橡胶树树根组织培养外植体消毒研究[J].安徽农业科学,2011,39(17):10127-10129,3.基金项目
海南省自然科学基金项目(30821) （30821）
中国热带农业科学院橡胶研究所基本科研业务费项目(XJSYWFZX2008-02). （XJSYWFZX2008-02）
