安徽农业科学2011，Vol.39Issue(17)：10133-10134,2.
黄粉虫酪氨酸羟化酶基因的克隆与序列分析
Molecular Cloning and Sequences Analysis of the Tyrosine Hydroxylase Gene in Mealworm, Tenebrio molitor
刘晶晶 1韩宏岩 1许维岸1
作者信息
- 1. 苏州大学基础医学与生命科学学院,江苏,苏州,215123
- 折叠
摘要
Abstract
[ Objective ] The research aimed to clone tyrosine hydroxylase gene of Tenebrio molitor (TmTH) of Coleoptera insects. [ Method ]RACE and PCR methods of cDNA tail end was used to clone TmTH gene of Coleoptera insects, and bioinformatics analysis was carried out.[ Result] The overall length of TmTH cDNA was 2 103 bp, which including 1605 bp open reading frame (ORF), thus can encoding a protein of 534 amino acid residues, and the molecular weight and pI of TmTH were 60.66 kD and 5.70,respectively. Based on that, we constructed the phylogenetic tree of TmTH. [ Conclusion] Molecular cloning of the tyrosine hydroxylase gene in mealworm, Tenebrio molitor can provide reference for further research of tyrosine hydroxylase of Coleoptera insects.关键词
黄粉虫/酪氨酸羟化酶/基因克隆/系统进化树Key words
Tenebrio molitor/Tyrosine hydroxylase/Gene cloning/Phylogenetic tree分类
农业科技引用本文复制引用
刘晶晶,韩宏岩,许维岸..黄粉虫酪氨酸羟化酶基因的克隆与序列分析[J].安徽农业科学,2011,39(17):10133-10134,2.
