安徽农业科学2011，Vol.39Issue(17)：10139-10142,10146,5.
药用野生稻(CC)-栽培稻(AA)异源单体附加系MAAL8的遗传分析
Genetic Analysis of Monosomic Alien Addition Line MAAL8 of O. officinalis ( CC ) -O. sativa ( AA )
摘要
Abstract
[ Objective ] By carrying out the anther culture on the monosomic alien addition line MAAL8 of O. officinalis-O, sativa and the back crossing with O. sativa H1493, the genetic characteristic of monosomic alien addition line was studied. [ Method] The phenotype analysis was used to study the separation proportion of progeny. Moreover, SSR and methylation analysis were used to study the transmission behavior of alien chromosome. [ Result] 78 plants of 145 backcross progenies preserved the rolled leaf mark trait of MAAL8. In 32 anther culture plants, 5 plants had the normal rolled leaves, and 2 plants had the extremely rolled leaves. The rest had the flat leaves. 14 couples of SSR markers were used to analyze, and it indicated that all the rolled leave plants could obtain the characteristic bands of O. officinalis, but the flat leave plants showed none of them. 11 polymorphic RFLP markers were used to carry out Methylation-Sensitative Southern analysis. It showed that the methylation variation manners of 8 plants between AA and CC genome were different. [ Conclusion] The alien chromosome of MAAL8 could independently and completely pass to the future generations via the meiosis, and the phenotype cha racteristic didnt change. Moreover,the methylation manner of O. officinali could be stably inherited in the hybrid progeny as the addition of single chromosome. The stability of methylation might have the certain effect on the relatively independent inheritance of alien chromosome in the genome environment of O. sativa.关键词
药用野生稻/异源单体附加系/花药培养/甲基化变异Key words
Oryza officinali/ Monosomic alien addition line/Anther culture/ Methylation variation分类
农业科技引用本文复制引用
刘如亮,刘虹,陈雁,覃瑞,李刚..药用野生稻(CC)-栽培稻(AA)异源单体附加系MAAL8的遗传分析[J].安徽农业科学,2011,39(17):10139-10142,10146,5.基金项目
湖北省自然科学基金项目(2008CDB392) （2008CDB392）
中南民族大学自然科学基金项目(yzz08005). （yzz08005）
