安徽农业科学2011，Vol.39Issue(17)：10157-10159,10166,4.
显齿蛇葡萄离体快繁体系的建立
Establishment of Raoid Prooagation System in vitro of Ampelopsis grossedentata
摘要
Abstract
[ Objective ] The research aimed to establish the fast and efficient of Ampelopsis grossedentata rapid propagation system by using the tissue culture method. [ Method] Ampelopsis grossedentata stem with the bud was the material, and the influences of different sterilization methods, media and hormone combinations on the rapid propagation in vitro of Ampelopsis grossedentata were studied. [ Result ] The explant sterilization effect of 75 ％ ethanol dipping 20 s + 0.1％ HgCl2 treating 8 min + Tween-80 1 - 2 drop was better. The optimal medium for axillary bud induction was B5 + 1.00 mg/L BA + 0.05 mg/L NAA. The optimal multiplication medium was MS/B5 + 1.50 mg/L BA + 0.05 mg/L NAA. 1/2MS + 0. 50 mg/L IBA was the best medium for rooting. [ Conclusion ] The high frequency occurrence system of Ampelopsis grossedentata rapid propagation in vitro was established. It laid the technology basis for the callus regeneration system, genetic transformation and clonal mutation screening.关键词
显齿蛇葡萄/外植体/离体培养Key words
Ampelopsis grossedentata/ Explant/ Isolated culture分类
农业科技引用本文复制引用
张霞,杨朝东,倪德江..显齿蛇葡萄离体快繁体系的建立[J].安徽农业科学,2011,39(17):10157-10159,10166,4.基金项目
农业部农业结构调整重大技术研究专项(06-08-02B). （06-08-02B）
