安徽农业科学2011，Vol.39Issue(17)：10161-10163,3.
绿玉树茎段组织培养再生体系的建立
Establishment of Tissue Culture Regeneration Systems of Stem Segments of Euphorbia tirucalli
摘要
Abstract
[ Objective ] The aim was to study the conditions of tissue culture regeneration seedling by using the stem segments of Euphorbia tirucalli and determine the optimum culture condition of each culture stage ,so as to provide references for the factory production and relative study of tissue culture seedling of E. tirucalli. [ Method] Taking the stem segments of E. tirucalli as explants,the effects of various mediums on germination rate,multiplication coefficient and rooting rate were studied. [ Result] The optimum induction medium of germination culture was 1/2MS +0.02 mg/L NAA + 1.00 mg/L 6-BA, with differentiation rate of 89.7％ ;the best subculture medium was 1/2MS + 0.02 mg/L NAA + 0.60 mg/L 6-BA + 3.00 mg/L AD, with multiplication coefficient of 5.70 ;the optimum rooting culture medium was 1/2MS + 0. 40 mg/L NAA + 0. 40 mg/L IBA ,with rooting rate of 100％ and transplanting survival rate of 80％. [ Conclusion] The tissue culture conditions of stem segments of E. tirucalli were determined primarily.关键词
绿玉树/茎段/组织培养Key words
Euphorbia tirucalli/Stem segment /Tissue culture分类
农业科技引用本文复制引用
刘召亮,何觉民,陈彪,梁钾贤..绿玉树茎段组织培养再生体系的建立[J].安徽农业科学,2011,39(17):10161-10163,3.基金项目
广东省科技攻关计划项目(2009B0203030092006B20201007). （2009B0203030092006B20201007）
