重庆医学2011，Vol.40Issue(28)：2812-2814,封2,4.DOI:10.3969/j.issn.1671-8348.2011.28.002
白藜芦醇联合化疗药物抑制胃癌SGC-7901细胞增殖作用研究
The experiment study on the effects and mechanisms of resveratrol combined with As2O3 and 5-FU in inducing apoptosis and inhibiting proliferation of SGC-7901 cell
张红 1周静 1姜政2
作者信息
- 1. 重庆医药高等专科学校临床医学系,401331
- 2. 重庆医科大学附属第一医院消化科,400016
- 折叠
摘要
Abstract
Objective To investigate the effects and mechanisms of Resveratrol (Res) combined with 5-FU on the proliferation and apoptosis of the gastric cancer lines SGC-7901 ,to provide experimental and theoretical foundation in clinical tumortherapies of Res.Methods Having processed SGC-7901 cell with a series of concentration including 5-FU alone or combined with Res for 211 h,48 h and 72 h respectively,the inhibition rate of cell proliferation were detected at different periods by methyhhiazdyl tetrazolium ( MTT) method , and the effective concentration fo 5-FU alone or 5-FU combined with Res for inhibition of cell proliferation was cal culated.Results The IC50 was 11.44[ μg/mL when SGC-7901 cell was processed by 5-FU alone,while it was 6.65 μg/mL when both 5-FU and 150 μmol/L of Res were used.It was shown that there was synergistic effect when Res was used combined with 5-FU.The apoptosis rate of SGC7901 cells in Res combined with 5-FU group (15.68％) was higher than that with 5FU alone (11.60％ ) (P＜0.05) .Karyopycnosis , nuclear chromosomal condensation and segmentation were observed in Res combined with 5-FU group by TUNFL.Conclusion Res could significantly intensified the effects of 5-FU in inhibiting the proliferation, inducing and promoting apoptosis of tumor cell and there was synergistic effect when Res was combined with 5-FU.关键词
细胞增殖/细胞凋亡/白藜芦醇/SGC-7901细胞Key words
cell proliferation / apoptosis / resveratrol : SGC-7901 cell引用本文复制引用
张红,周静,姜政..白藜芦醇联合化疗药物抑制胃癌SGC-7901细胞增殖作用研究[J].重庆医学,2011,40(28):2812-2814,封2,4.
