工业微生物2011，Vol.41Issue(4)：6-9,4.DOI:10.3969/j.issn.1001-6678.2011.04.002
紫外诱变原生质体选育低温碱性脂肪酶高产菌株
Breeding of cold-adapted and alkaline lipase producing mutants by protoplast mutagenesis of ultraviolet irradiation
摘要
Abstract
The cold-adapted and alkaline lipase producing strain Acinetobacter johnsonii LP28 was selected as the parent strain and the protoplast was prepared with ED TA and lysozyme . The corresponding values usad were 0.15mg/mL lysozyme , at 37℃ for 45 min . The rate of cell oonverted to protoplast was 90％ and the regeneration efficiency was about 0.9％ . Acinetobactrr johnsonii LP28 was induced by protoplast mutagenesis of ultraviolet irradiation and the mutant P169 was obtained . Its lipase activity was as high as 5.16 U/m L , increased by 2.19 tmes in comparison with that of the Acinetobacter johnsonii LP28 .关键词
脂肪酶/原生质体/紫外诱变Key words
lipase / protoplast/ ultraviolet mutagenesis引用本文复制引用
戚薇,魏玉洁,颜虎,张杰,王海宽..紫外诱变原生质体选育低温碱性脂肪酶高产菌株[J].工业微生物,2011,41(4):6-9,4.基金项目
天津市自然科学基金资助项目(09JCZDJC17800) （09JCZDJC17800）
