重庆医学2011，Vol.40Issue(28)：2848-2850,2853,4.DOI:10.3969/j.issn.1671-8348.2011.28.016
三种肝保存液低温保存生物人工肝用C3A细胞的效果比较
The effect comparison of hypothermic UW,Celsior and HTK solution for the storage of C3A hepatocytes
摘要
Abstract
Objective To compare the effect of University of Wisconsin solution(UW) ,Celsior solution(CS) and Histidine-tryp tophan-ketoglutarate solution( HTK)for the storage of C3A hepatocytes at 4 ℃ .Methods C3A hepatocytes were divided into the following three groups : UW group,CS group,and HTK group.After 72 h of hypothermic storage, the cell viability and death rate ( Flow Cytometry) , the alanine aminotransferase( ALT) release and lactic acid production, and the ability of hepatocytes to synthesize urea and secrete albumin were respectively measured.Results Compared with that in HTK group,the cell viability(78.56％ ±4.67％ vs 76.03％ ±3.53％ , 60.5％ ±3.18％ ,P＜0.05)of C3A hepatocytes which were stored for 72 h were significantly improved in UW and CS groups.The ALT release and the lactic acid production ( P ＜ 0. 05) were significantly suppressed in UW and CS groups.The ability of hepatocytes to synthesize urea[ ( 0.52±0.11) mmol/L vs ( 0.51±0.06) mmol/L , (0.32+0.05) mmol/L , P＜0.05]and secrete albumin[(l.79±0.26) g/L vs (1.75 ± 0.21) g/L, (1.20 ± 0.17) g/L, P ＜ 0.05 ] were better maintained in UW and CS groups.There was no difference between UW and CS group for the effect of storage of C3A hepatocytes. Conclusion Com pared with HTK solution,the hypothermic storage(4 ℃ )of C3A hepatocytes in UW or CS solution could obviously improve the cell viability rate,decrease the ALT release and the lactic acid production,and to protect the ability of hepatocytes to synthesize urea and secrete albumin.关键词
肝,人工/UW液/Celsior液/HTK液Key words
liver , artificial/ university of wisconsin solution / celsior solution / histidine-tryptophan-ketoglutarate solution引用本文复制引用
林虎,李庆勇,蒋知新,张鋆歆,彭凌,杜平,张清华,高毅..三种肝保存液低温保存生物人工肝用C3A细胞的效果比较[J].重庆医学,2011,40(28):2848-2850,2853,4.基金项目
全军医学科研"十一五"计划专项课题(08Z017) （08Z017）
国家高技术研究发展计划(863计划,2006AA02A141). （863计划,2006AA02A141）
