重庆医学2011，Vol.40Issue(28)：2862-2864,3.DOI:10.3969/j.issn.1671-8348.2011.28.021
医院患者HIV抗体筛查阳性与免疫印迹试验结果对比分析
Comparative analysis of HIV antibody screening positive and western blot test results in hospital patients
谭兵 1詹廷西 1李青 1李成 1吕莉 1邢燕 1张国珍1
作者信息
- 1. 重庆医科大学附属第一医院输血科,400016
- 折叠
摘要
Abstract
Objective Comparing and analyzing the HIV antibody screening positive results and Western Blot test results, to study the relationship between screening positive results and Western Blot results. Methods According to the "National Guideline for Detection of HIV/AIDS", positive samples screened by HIV antibody screening test were confirmed by Western Blot test. Results Among 287 positive samples tested by primary screening test,251 samples were confirmed to be positive(87.46％) ,16 sampies indeterminate(5.57％)and 20 samples negative(6.97％) by WB test. The positive coincidence rates of ELISA/Electroselenium method and WB were 89.96％ and 90.29％ respectively. The mean value of S/CO were 16.93、6. 996 and 5. 568 in confirmatory positive group, indeterminate goup and negative group, respectively. When the S/CO value of the positive samples screened by ELISA equal to or more than 1 and less than 6,18.75 ％ were confirmed by WB, and when equal to or more than 6 and less than 10,69.23 ％ were confirmed by WB, and when more than 10,97.05 ％ were confirmed by WB. The appearance rate of gp160、 gp120、gp41、 p66、 p24 reactive bands were all over 95 ％ in WB confirmed HIV-1 antibody positive samples. Conclusion With the S/CO values increased, the positive coincidence rate of ELISA and WB increased, but ELISA results with high S/CO values does not certainly mean HIV infection. The final HIV antibody test results must be confirmed by WB test as some false positives do exist in the screening test. WB test has limitation in indeterminate samples.关键词
HIV抗体/酶联免疫吸附测定/免疫印迹法Key words
HIV antibodies/enzyme-linked immunosorbent assay/immunoblotting引用本文复制引用
谭兵,詹廷西,李青,李成,吕莉,邢燕,张国珍..医院患者HIV抗体筛查阳性与免疫印迹试验结果对比分析[J].重庆医学,2011,40(28):2862-2864,3.
