实用医学杂志2011，Vol.27Issue(17)：3109-3112,4.DOI:10.3969/j.issn.1006-5725.2011.17.007
沉默Medl9对人乳腺癌裸鼠成瘤的影响
Influence of silencing Med19 on the tumor formation of human breast cancer cells in nude mice
摘要
Abstract
Objective To study the influence of silencing Med19 by lentivirus-mediated RNA interference on the tumor growth of human breast cancer cells in nude mice . and to investigate the effects of Med19 on the oncogenesis and progression of breast cancer. Methods Human breast cancer MCF-7 cells steady infected with lentiviruses delivering small interfering RNA ( siRNA) against Med19 gene had been constructed. Western blotting was used to detect the silence of Med19 protein. Then the MCF-7 cells were injected subcutaneously into the back of BALB/c nude mice ( KD group). Tumorigenicity and the growth of transplanted tumor in nude mice were observed. Growth curves were plotted based on mean tumor volume in each experimental group at the indicated time points. Meanwhile , the negative control group ( NC group) which MCF-7 cells steady infected with lentiviruses delivering negative control sequence was set up. Results The constructed Med19-siRNA lentivirus vector could suppress the expression of Med19 protein in MCF-7 cells effectively, and the level of Med19 protein was decreased by 62％. As compared with that in NC group ,the tumor growth velocity in KD group was slower (t = 4.843, 6.291, 5.317, respectively, all P ＜ 0.01). The final weights of tumor in KD group and NC group were (0.20 ± 0.18) g and (0.67 ± 0.27)g, respectively; and the final volume were ( 620.08 ± 263.07 ) mm3 and ( 1406.77 ± 351.14)mm3 , respectively (t = 4.399 , 3.615 , respectively , all P ＜ 0.01). Conclusion The silencing Med19 gene can strongly suppress the tumor formation and transplanted tumor growth of human breast cancer in nude mice . which suggests that Med19 may play an important role in the oncogenesis and progression of breast cancer , and may constitute a potential therapeutic target in this disease.关键词
乳腺肿瘤/Med19/RNA干扰/慢病毒Key words
Breast neoplasms/ Med19/ RNA interference/ Lentivirus引用本文复制引用
王晓艳,华东,李莉华,高琪,郭子健..沉默Medl9对人乳腺癌裸鼠成瘤的影响[J].实用医学杂志,2011,27(17):3109-3112,4.基金项目
无锡市卫生局项目(编号:XM1012) （编号:XM1012）
