安徽农业科学2011，Vol.39Issue(19)：11374-11376,11379,4.
黑曲霉葡萄糖苷酶的分离纯化及酶学性质研究
Study on Separation and Purification of β-glucosidase from Aspergillus niger and its Enzymology Character
摘要
Abstract
[ Objective ] The study aimed to isolate the exocellular β-glucosidase from Aspergillus niger that could hydrolyze the swertiamarin and research its molecular weight, optimal catalytic temperature, optimal pH, stability and catalytic characteristics. [ Method ] The A. niger liquid was fermented for 3 d and then its culture liquid was collected. After the culture liquid was made through the salting out and column separation, a β-glucosidase was gained. [ Result] The β-glucosidase had the molecular weight of 86 kD, the optimal pH of 5.0 -6.0, the optimal reaction temperature of 50 -60 ℃ and showed stable at below 60 ℃. The Ca2+ ,Zn2+ , Fe3+ and Cu2+ could restrain the activity of the βglucosidase and the Mg2+ and Mn2+ could activate its activity. Cellobiose was the optimal catalytic substrate of β-glucosidase. [ Conclusion] This enzyme, similar to the most A. niger from the fungi, showed the weak affinity to the swertiamarin and the strong affinity to p-NPG.关键词
黑曲霉/β-葡萄糖苷酶/分离/纯化Key words
Aspergillus niger/ β-glucosidase/ Isolation/ Purification分类
农业科技引用本文复制引用
常军,周斌,胡娜..黑曲霉葡萄糖苷酶的分离纯化及酶学性质研究[J].安徽农业科学,2011,39(19):11374-11376,11379,4.基金项目
江西科技师范学院博士科研启动金项目 （）
南昌市教育厅科技项目(GJJ10593) （GJJ10593）
天津市自然科学基金项目(10JCZDJC21400). （10JCZDJC21400）
