安徽农业科学2011，Vol.39Issue(19)：11474-11476,3.
一株淮山炭疽病拮抗放线菌的分离及初步鉴定
Isolation of an Antagonistic Actinomycete Strain against Dioscorea opposita Thunb Anthracnose and Its Primary Identification
摘要
Abstract
[Objective]The paper was to isolate actinomycete strains with antagonistic activity against Dioscorea opposita Thunb anthracnose from the rhizosphere soil of medicinal plants in Hainan Province, and identify its taxonomic status. [ Method ] Using HV medium, actinomycete strains were isolated from the rhizosphere soil of frangipani (Plumeria rubra L. ). The active antagonistic actinomycetes were selected by using confrontation culture method and inhibition test of fermentation products. According to morphology,combined with 16S rRNA gene analysis,the taxonomic status of active actinomycetes was identified. [ Result]Twelve strains of actinomycetes were isolated from the rhizosphere soil of medicinal plant frangipani(P. rubra L. ). Strain 51173 showed significant antifungal activity against Colletotrichum gloeosporioides, which was identified to be a member of Streptomyces violaceusniger 16S rRNA gene clade. [ Conclusion] Strain 51173 showed a good application potential for biological control against D. opposita Thunb anthracnose.关键词
淮山/炭疽病/放线菌/拮抗Key words
Dioscorea opposita Thunb/Anthracnose/Actinomycetes/Antagonistic activity分类
农业科技引用本文复制引用
黄小龙,曹树威,周双清,黄东益,汤维..一株淮山炭疽病拮抗放线菌的分离及初步鉴定[J].安徽农业科学,2011,39(19):11474-11476,3.基金项目
海南大学青年基金项目(qnjj1018) （qnjj1018）
海南省作物学重点学科资助项目. （）
