中国病理生理杂志2011，Vol.27Issue(10)：1902-1906,5.DOI:10.3969/j.issn.1000-4718.2011.10.009
NKX3.1下调前列腺癌PC-3细胞中抗凋亡基因bcl-2的表达
NKX3.1 down-regulates bcl-2 gene expression in prostate cancer PC-3 cells
摘要
Abstract
AIM: To investigate the effect of NKX3. 1 on the gene expression of bcl - 2 and apoptosis in prostate cancer PC -3 cells. METHODS: The eukaryotic expression plasmid pcDNA3. 1 - NKX3. 1 was transiently transfected into PC -3 cells. RT - PCR and Western blotting were used to detect the effects of NKX3. 1 on the expression of bcl -2 gene. Down - regulatory effect of NKX3. 1 on bcl - 2 gene was detected by EMSA. Flow cytometry and apoptotic body staining were carried out to study the effects of NKX3. 1 on apoptosis of PC -3 cells. RESULTS: The mRNA and protein expression of bcl -2 in PC - 3 cells was down - regulated by over - expression of NKX3. 1. The EMSA result showed that NKX3. 1 interacted with the NKX3. 1 binding elements in upstream regulatory region of bcl -2 gene. The results of flow cytometry showed that the number of apoptotic PC - 3 cells increased by 1. 41 - fold after NKX3. 1 transfection to PC - 3 cells. NKX3. 1 increased the apoptotic bodies stained by Hoechst 33258 significantly. CONCLUSION: NKX3. 1 down -regulates the expression of anti - apoptotic gene bcl -2 and induces the apoptosis of prostate cancer PC -3 cells.关键词
NKX3.1蛋白/PC-3细胞/基因,bcl-2/细胞凋亡Key words
NKX3. 1 protein/PC -3 cells/Genes,bcl-2/Apoptosis分类
医药卫生引用本文复制引用
陈兆波,刘春艳,倪娜娜,于洋,张鹏举,陈蔚文,崔福爱,姜安丽..NKX3.1下调前列腺癌PC-3细胞中抗凋亡基因bcl-2的表达[J].中国病理生理杂志,2011,27(10):1902-1906,5.基金项目
国家自然科学基金资助项目(No.81071720) （No.81071720）
山东省优秀中青年科学家科研奖励基金计划资助项目(No.BS2009SW042) （No.BS2009SW042）
山东省科技发展计划资助项目(No.2009GG20002045) （No.2009GG20002045）
