中国组织工程研究与临床康复2011，Vol.15Issue(36)：6718-6721,4.DOI:10.3969/j.issn.1673-8225.2011.36.016
胃癌肿瘤干细胞的分离与成脂分化
Isolation and aipogenic differentiation of gastric cancer stem cells
匡弢 1王雷 2宋文 3孟冬梅4
作者信息
- 1. 青岛大学医学院附属医院,特需保健科,山东省青岛市,266003
- 2. 青岛大学医学院附属医院,青岛市立医院血液科,山东省青岛市,266071
- 3. 青岛大学医学院附属医院,放射科,山东省青岛市,266003
- 4. 青岛大学医学院附属医院,青岛市常见病重点实验室-痛风病实验室,山东省青岛市,266003
- 折叠
摘要
Abstract
BACKGROUND: Whether cancer stem cells are present in all tumors is still controversial. OBJECTIVE: To isolate and identify cancer stem cells from human gastric cancer cells, and to explore the mechanism of gastric cancer. METHODS: Gastric cancer cells were primarily cultured and CD44 and CD29 positive expression rates were detected by flow cytometry to sort positive cells for adipogenic induction. RESULTS AND CONCLUSION: CD44 and CD29 positive percentages in gastric cancer cells were 5.67% and 5.53%, respectively, indicating that CD44 and CD29 positive cells had an adipogenic capacity. It is proved that there are tumor stem cells with differentiation capacity in gastric cancer cells.关键词
肿瘤干细胞/胃癌细胞/成脂分化/生物学性状/CD44/CD29分类
基础医学引用本文复制引用
匡弢,王雷,宋文,孟冬梅..胃癌肿瘤干细胞的分离与成脂分化[J].中国组织工程研究与临床康复,2011,15(36):6718-6721,4.
