中国组织工程研究与临床康复2011，Vol.15Issue(36)：6727-6731,5.DOI:10.3969/j.issn.1673-8225.2011.36.018
不同浓度肝细胞生长因子及表皮细胞生长因子体外联合诱导兔骨髓间充质干细胞向肝细胞分化
In vitro combination of different-concentration hepatocyte growth factor and epidermal growth factor induces differentiation of rabbit bone marrow mesenchymal stem cells into hepatocytes
摘要
Abstract
BACKGROUND: Studies have confirmed that in a variety of micro-environments, bone marrow mesenchymal stem cells (BMSCs) can be induced to differentiate into mature hepatocytes, but the inducible condition and differentiating rate are inconclusive. To select the appropriate inducer and its concentration is particularly important. OBJECTIVE: Through study on the differentiation of rabbit BMSCs into hepatocytes in vitro induced by different concentrations of hepatocyte growth factor (HGF) and epidermal cell growth factor (EGF), to find the best concentration combination of HGF and EGF. METHODS: BMSCs were induced with different concentrations of HGF and HGF. The morphological changes of cells were observed. In different culture periods, the hepatic surface phenotype including alpha fetoprotein (AFP), albumin (ALB) and synthesis function of hepatocytes were determined. RESULTS AND CONCLUSION: After prolonged culture, hepatocyte-like cells were seen. Until day 7, AFP was positive, but reduced in the following days (P < 0.05), and after that, there were no difference among all groups (P > 0.05). Until day 14, ALB expression was positive, and increased with the culture time (P < 0.05). The higher the concentration of cell growth factors, the higher the number of positive cells (P < 0.05). The concentration of ALB in the supernatant in the early stage (9-15 days) showed a concentration-dependent manner, and reached the peak at 18 or 21 days. Then the concentration began to decreased, and there were no difference (P > 0.05). The high concentrations of HGF and EGF may enhance the rate of differentiation, and the best combination is HGF 60 ug/L and EGF 4.5 mg/L.关键词
肝细胞生长因子/骨髓间充质干细胞/肝细胞/表皮细胞生长因子/诱导/分化/兔分类
基础医学引用本文复制引用
罗伟,肖恩华,罗建光,尚全良,伍玉枝,李艳辉,谭艳..不同浓度肝细胞生长因子及表皮细胞生长因子体外联合诱导兔骨髓间充质干细胞向肝细胞分化[J].中国组织工程研究与临床康复,2011,15(36):6727-6731,5.基金项目
国家自然科学基金(30070235、30470508、30870695),湖南省自然科学基金重点项目(06JJ20081),湖南省自然科学基金项目(07JJ6040),长沙市科技计划重点项目(K071532-11). （30070235、30470508、30870695）
