中国组织工程研究与临床康复2011，Vol.15Issue(39)：7288-7291,4.DOI:10.3969/j.issn.1673-8225.2011.39.017
快速建立小牛椎体骨质疏松模型
Rapid establishment of a calf vertebral osteoporosis model
曹涌 1张烽 1姚羽 1邱军 1陈向东 1赵剑1
作者信息
- 1. 南通大学附属医院脊柱外科,江苏省南通市226001
- 折叠
摘要
Abstract
BACKGROUND: C ur entry, osteo p orosis anim al models used for biomechanical studies of spinaI osteoporosis are ovariectomized animal models or drug-induced animal models, and they have many problems such as model establish merut for a long time and poor reliability.OBJECTIVE: To estableh an animal model of osteoporotic vertebras rapidly by decalcifying fresh calf lumbar vertebras with Shandon TBD-1.METHODS: Twenty-four calf lumbar vertebrae from four fresh calves were classified into three groups: in pre-experrnent group, the vertebrae were immersed in Shandon TBD-1; in decalcified group, the vertebrae were decalcified by using Shandon TBD-1; h control group, no decalcification was done. The vertebrae were subjected to DEXA to measure bone mineral density (BMD). And then two pedicle screws were drhren into calf lumbar vertebrae. After that, the maximum axial pullout strength was recorded. RESULTS AND CONCLUSION: The BMD of the vertebrae in the pre-experiment group was decreased with the time of decalcification. The BMD and the maximum axial pullout strength in the decalcified group we re signrficanthy lower than those in the control group (P < 0.01). And there was a linear positive correlation between the BMD and the maximum axial pullout strength. An animal model of osteoporotic vertebra can be established rapidly in calf by means of decalcifying fresh calflumbar vertebras wth Shandon TBD-1.关键词
骨质疏松/脱钙骨体外模型/生物力学/骨密度/动物模型分类
医药卫生引用本文复制引用
曹涌,张烽,姚羽,邱军,陈向东,赵剑..快速建立小牛椎体骨质疏松模型[J].中国组织工程研究与临床康复,2011,15(39):7288-7291,4.
