中国组织工程研究与临床康复2011，Vol.15Issue(32)：5913-5917,5.DOI:10.3969/j.issn.1673-8225.2011.32.006
辛伐他汀体内给药对大鼠骨量和骨髓基质干细胞增殖分化的影响
Effects of simvastatin on bone mass and bone marrow mesenchymal stem cells proliferation in rats
摘要
Abstract
BACKGROUND: As a widely used lipid-lowering drug, simvastatin has been rarely reported addressing its effects on bone metabolism and proliferation and differentiation of bone marrow stromal cells (BMSCs).OBJECTIVE: To investigate the effects of simvastatin on bone mass and differentiation and proliferation of BMSCs in rats, as well as the expression levels of Smadi, 2, 7 mRNA.METHODS: Sixteen 6-week-old female Sprague-Dawley rats were randomized into two groups of eight animals each: simvastatin group, administered daily with 20 mg/kg simvastatin by gavage for 9 weeks; control group, administered with distilled water for 9 weeks. All animals were sacrificed one day after the final administration. The left femora were removed for the measurement of bone histomorphometry and bone mineral density (BMD). BMSCs derived from the right femora and tibiae were cultured in osteoblastic differentiation-induced medium. Cell Counting Kit-8 (CCK-8) was used to assay the proliferation of BMSCs. Alkaline phosphatase (ALP) activity, ALP staining were performed on the 16th day and von Kossa staining on the 28th day. Real-time RT-PCR was used to evaluate the expression levels of mRNA of Smadi, 2, 7 at the 21st day. RESULTS AND CONCLUSION: After being administrated with simvastatin for 9 weeks, the bone mass and BMD of rats had no significant change. The expression level of mRNA of Smadi, 2, 7 showed no significant change, so were the results of ALP activity, positive cell rate of ALP staining, von Kossa staining, and the proliferation of BMSCs. Administration with 20 mg/kg simvastatin for 9 weeks had no significant effect on bone mass and the differentiation and proliferation of BMSCs in rats, so were the expression levels of Smadi, 2, 7 mRNA.关键词
辛伐他汀/骨组织形态计量学/骨密度/骨髓基质干细胞/Real-time RT-PCR分类
医药卫生引用本文复制引用
梁春雨,田发明,张辉,张柳,张英泽..辛伐他汀体内给药对大鼠骨量和骨髓基质干细胞增殖分化的影响[J].中国组织工程研究与临床康复,2011,15(32):5913-5917,5.基金项目
百名优秀创新人才支持计划 （）
河北省自然科学基金项目项目(C2006000580). （C2006000580）
