中国组织工程研究与临床康复2011，Vol.15Issue(32)：5923-5927,5.DOI:10.3969/j.issn.1673-8225.2011.32.008
全骨髓贴壁法分离培养大鼠骨髓间充质干细胞及其生物学特性
Culture condition and biological characteristics of rat bone marrow mesenchymal stem cells by using the whole bone marrow adherence method
摘要
Abstract
BACKGROUND: Bone marrow mesenchymal stem cells (BMSCs) are widely used as seed cells in tissue engineering and otherfields. Therefore, well growth and enough number of rat BMSCs are important for above-mention studies.OBJECTIVE: To explore the suitable primary culture condition of rat BMSCs in vitro, and to observe the growth property ofBMSCs.METHODS: BMSCs were separated using whole bone marrow culture method and purified using attachment method.Morphology and growth characteristics were observed under inverted phase contrast microscope and cytoskeleton was detectedby immunofluorescence. Growth curve was drawn using MTT method. Expression of BMSC surface antigens was detected byflow cytometer.RESULTS AND CONCLUSION: After isolation and culture, cells developed a spindle or polygonal appearance. Growth curvewas S-shaped, and the cultured BMSCs possessed normal morphology and cytoskeleton. At the third passage, BMSCs werepositive forCD29, CD44, CD71, CD90, but negative for CD13, CD34, CD45, CD133. Cells isolated and cultured by theadherence separation method in this study are identified as rat BMSCs by morphology and surface antigens, which have thebiological characteristics of BMSCs.关键词
骨髓间充质干细胞/大鼠/贴壁筛选法/分离培养/生物学特性分类
基础医学引用本文复制引用
赵林,冯智慧,焦淑贤,李南南..全骨髓贴壁法分离培养大鼠骨髓间充质干细胞及其生物学特性[J].中国组织工程研究与临床康复,2011,15(32):5923-5927,5.基金项目
青岛市科技发展计划(No:09-1-1-25-nsh),课题名称:骨髓基质干细胞对失代偿性病毒性肝炎修复的中长期影响. （No:09-1-1-25-nsh）
