中国组织工程研究与临床康复2011，Vol.15Issue(32)：5945-5950,6.DOI:10.3969/j.issn.1673-8225.2011.32.013
残粒脂蛋白激活过氧化物酶体增殖物激活受体γ诱导人脂肪间充质干细胞的成脂分化
Remnant-like particles activate peroxisome proliferator-activated receptor gamma and induce adipogenic differentiation of human adipose mesenchymal stem cells
摘要
Abstract
BACKGROUND: Remnant-like particles (RLPs) can induce rat adipose-derived mesenchymal stem cells (ADSCs) todifferentiate into mature adipocytes.OBJECTIVE: To explore the effects of RLPs on the adipogenic differentiation of human ADSCs and on mRNA expressions ofperoxisome pro life rat or-activated receptor , (PPAR-α) and adiponectin (APN).METHODS: After stimulation by standard cocktail inducer, lipid droplets within adipocytes were identified by oil red O staining.Human RLPs were isolated by density gradient ultracentrifugation and immuno-affinity chromatography from postprandial plasmaof hypertriglyceridemic patients at 4 hours after a high-fat meal. ADSCs were stimulated by 10 mg/L insulin and differentconcentrations of RLPs (0, 50, 100, 150, 200 mg/L).RESULTS AND CONCLUSION: After stimulation at 12 days, a small amount of lipid droplets was found in the control group or50 mg/L RLPs stimulated ADSCs by oil red O staining. Lipid droplets increased gradually with the increase of RLPsconcentrations from 100 to 200 mg/L and reached the peak in 200 mg/L RLPs stimulated ADSCs. The mRNA expressions ofPPAR-α And APN increased with the increase of RLPs concentrations from 50-200 mg/L (P < 0.05). APN mRNA expression in200 mg/L RLPs group was significantly weaker than that in 150 mg/L RLPs group (P < 0.05), while it showed no significantdifference when compared with that in 100 mg/L RLPs group. It indicates that RLPs induce adipogenic differentiation of humanADSCs through activating PPAR, and up-regulate APN expression.关键词
残粒脂蛋白/氧化物酶体增殖物激活受体γ(PPAR-γ)/脂肪间充质干细胞/成脂分化/脂联素分类
基础医学引用本文复制引用
马美芳,文铁,刘玲..残粒脂蛋白激活过氧化物酶体增殖物激活受体γ诱导人脂肪间充质干细胞的成脂分化[J].中国组织工程研究与临床康复,2011,15(32):5945-5950,6.基金项目
中华医学会动脉粥样硬化研究专项资金课题项目(09010570212) （09010570212）
湖南省杰出青年基金项目(10JJ1008) （10JJ1008）
中南大学湘雅二医院代谢综合征研究中心资助课题项目(DY-2008-01-01). （DY-2008-01-01）
